發(fā)布時間：2018-06-21 04:54

  本文選題：銀納米材料 + 明膠　； 參考：《曲阜師范大學(xué)》2015年碩士論文

【摘要】：銀納米材料特別是銀納米簇(Ag NCs),具有良好的納米小尺寸效應(yīng)、熒光特性、以及過氧化物酶活性等,已成為當(dāng)今材料學(xué)、化學(xué)和生物學(xué)以及納米醫(yī)學(xué)的研究熱點之一,并被廣泛應(yīng)用于生物標(biāo)記、細(xì)胞成像、酶催化以及重金屬離子與生物小分子傳感檢測等領(lǐng)域。本論文主要從以下幾個方面進(jìn)行了研究:(一)以具有溶膠-凝膠轉(zhuǎn)換特性的明膠為穩(wěn)定劑和還原劑,一鍋式反應(yīng)合成了具有過氧化物酶活性和高穩(wěn)定性的銀納米顆粒。通過在常見酶促反應(yīng)體系中各種金屬離子對其催化性能的考察,證實了汞離子能夠顯著增強(qiáng)銀納米顆粒的催化活性。應(yīng)用96孔板實現(xiàn)了對復(fù)雜體系(血液和廢水)中汞離子高通量地測定,而且能借助明膠獨特的溶膠-凝膠轉(zhuǎn)換特性于常溫下穩(wěn)定地存儲。此外,該方法能避免傳統(tǒng)檢測方法如熒光法受復(fù)雜體系背景干擾的影響。(二)以谷胱甘肽和硫辛酸為穩(wěn)定劑和模板,合成了高熒光強(qiáng)度的銀納米簇。谷胱甘肽的引入,成功實現(xiàn)了金屬離子特異性識別的轉(zhuǎn)移,即加谷胱甘肽之前對汞離子響應(yīng)轉(zhuǎn)變?yōu)榧庸入赘孰闹�后對銅離子響應(yīng),同時伴隨著顏色變化,從而開發(fā)了一種熒光和比色雙重檢測銅離子的傳感器,并成功應(yīng)用于血液中銅離子的雙重檢測,此外,螯合劑乙二胺四乙酸的引入實現(xiàn)了銀納米簇的熒光恢復(fù)。將該納米簇與小鼠肌肉組織進(jìn)行培養(yǎng),可用于組織細(xì)胞的示蹤標(biāo)記。(三)利用明膠還原銀反應(yīng)并在合適時間借助低溫速凍終止反應(yīng),制備了包含銀納米顆粒與銀離子的明膠銀納米復(fù)合材料。研究表明,在堿性環(huán)境下該復(fù)合材料對甲醛具有高特異識別性能,銀納米顆粒催化甲醛還原銀離子反應(yīng)可引起銀納米顆粒的增長,實現(xiàn)復(fù)合材料顏色信號的顯著改變。同時,借助明膠的獨特溶膠-凝膠轉(zhuǎn)換特性,將銀復(fù)合材料穩(wěn)定固定于毛細(xì)管壁,進(jìn)而利用毛細(xì)現(xiàn)象自動吸取樣品,實現(xiàn)了對水產(chǎn)品中甲醛的簡便、靈敏、快速、可視化的比色分析。
[Abstract]:Silver nanomaterials, especially silver nanoclusters, such as small size effect, fluorescence characteristics and peroxidase activity, have become one of the hot research fields in materials science, chemistry, biology and nanomedicine. It has been widely used in biomarkers, cell imaging, enzyme catalysis and the detection of heavy metal ions and biological small molecules. In this paper, we have studied the following aspects: 1. Silver nanoparticles with peroxidase activity and high stability were synthesized by one-pot reaction using gelatin with sol-gel conversion as stabilizer and reductant. It is proved that mercury ion can significantly enhance the catalytic activity of silver nanoparticles by investigating the catalytic performance of various metal ions in the common enzymatic reaction system. The high throughput determination of mercury ions in complex systems (blood and wastewater) has been achieved by using 96 hole plates and can be stably stored at room temperature with the help of the unique sol-gel conversion characteristics of gelatin. In addition, this method can avoid the influence of complex system background interference on traditional detection methods such as fluorescence method. (2) Silver nanoclusters with high fluorescence intensity were synthesized with glutathione and lipoic acid as stabilizers and templates. The introduction of glutathione successfully achieved the transfer of metal ion specific recognition, that is, the response to mercury ion before glutathione was changed to copper ion after glutathione addition, and the color change was accompanied by the change of color. A dual detection sensor for copper ions was developed, which was successfully applied to the detection of copper ions in blood. In addition, the fluorescence recovery of silver nanoclusters was realized by the introduction of the chelating agent ethylenediamine tetraacetic acid. The nanoclusters were cultured with mouse muscle tissue and could be used for tracer labeling of tissue cells. (3) Gelatin silver nanocomposites containing silver nanoparticles and silver ions were prepared by reducing silver with gelatin and terminating at a suitable time by rapid freezing at low temperature. The results show that the composite has a high specific recognition ability to formaldehyde in alkaline environment. Silver nanoparticles catalyze the reduction of silver ions by formaldehyde can lead to the increase of silver nanoparticles and the significant change of color signals of the composite materials. At the same time, with the help of the unique sol-gel conversion characteristics of gelatin, the silver composite material was stably fixed on the capillary wall, and then the sample was automatically absorbed by capillary phenomenon, which realized the simplicity, sensitivity and rapidity of formaldehyde in aquatic products. Visual colorimetric analysis.
【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：O614.122;TB383.1

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本文編號：2047312