福建省begomoviruses的檢測和分子生物學研究
發(fā)布時間：2020-12-08 04:09
雙生病毒是一類重要的植物病毒,病毒粒子呈典型的孿生狀,基因組由單鏈環(huán)狀DNA構成。當前,雙生病毒科被分為9個屬,分別為Becurtovirus,Begomovirus,Capulavirus,Curtovirus,Eragrovirus,Grablovirus,Mastrevirus,Topocuvirus 和 Turncurtovirus。雙生病毒,特別是begomovirus,在熱帶和亞熱帶地區(qū)嚴重危害。在一些欠發(fā)達國家和地區(qū),它們甚至會造成饑荒。近年來,begomovirus的發(fā)生在中國南方越來越普遍。然而,這類病毒在福建省的發(fā)生情況還不甚清楚。在第一部分,本研究于2015-2017年間,從福建各地采集了疑似begomovirus侵染的朱槿、玫瑰茄、黃秋葵、黃麻、芒麻、番茄和勝紅薊等植物樣本。利用簡并引物PCR并結合必要的全基因組克隆,本研究從這些樣本中鑒定到6種begomovirus,分別為木爾坦棉花曲葉病毒（CLCuMuV）、番茄黃化曲葉病毒（TYLCV）、越南黃麻黃脈病毒（COYVV）,苧麻花葉病毒（RamV）、中國勝紅薊黃脈病毒（AYVCnV）和野葛花葉病毒（KuMV）。...
【文章來源】：福建農林大學福建省
【文章頁數】：115 頁
【學位級別】：博士
【文章目錄】：
List of Abbreviations
摘要
Abstract
Chapter 1 Introduction and Review of literature
1.1 Geminiviruses: General description
1.2 Geminiviruses:Txonmy
1.3 Geminiviruses: Genome organization
1.4 Geminiviruses: gene function and host-virus interaction
1.5 Geminiviruses: Ecology
1.6 Geminiviruses in China and Fujian Province
1.7 Objectives of Research
Chapter 2 Detection of Begomoviruses from different host plants in Fujian Province ofChina
2.1 Background
2.2 Materials and methods
2.2.1 Collection of samples
2.2.2 Genomic DNA extraction
2.2.3 PCR amplification, cloning, and sequencing
2.2.4 PCR/RCA amplification of DNA P
2.2.5 Phylogenetic analysis
2.3 Results and Discussion
2.3.1 Identification of Begomoviruses and Satellites in Fujian Province of China
2.3.1.1 Detection of Cotton leaf curl Multan virus and Betasatellite from Hibiscussabdariffa
2.3.1.2 Detection of Ramie mosaic virus （RamV） from Ramie （Boehmeria nivea）
2.3.1.3 Detection of Tomato yellow leaf curl virus （TYLCV） from Tomato plants（Lycopersicon esculentum L）
2.3.1.4 Detection of Corchorus yellow vein Vietnam virus （CoYVV） from Jute plants（Corchorus olitorius L.）
2.3.1.5 Detection of Ageratum yellow vein China virus （AYVCnV） infecting Ageratum（Ageratum conyzoides）
2.3.1.6 Detection of Kudzu mosaic virus virus （KuMV） infecting Kudzu （Ageratumconyzoides）
Chapter 3 Construction of Infectious clone and Molecular studies/characterization ofCLCuMuV, RamV and CoYVV
3.1 Background
3.2 Material and Methods
3.2.1 Collection of samples and DNA extraction
3.2.2 Construction of infectious clone and agrobacterium infilteration
3.2.3 Confirmation of infection by Southern Blot analysis
3.2.4 Cloning and sequence analysis:
3.3 Results and Discussion
Chapter 4 Molecular characterization and RSV Co-infect Nicotiana benthamiana withbegomoviruses
4.1 Background
4.2 Material and Methods
4.2.1 Virus Source and inoculation
4.2.2 Detection of TSSs by Southern Blot analysis
4.2.3 Primer Design
4.2.4 High Throughput sequencing of 5' ends of CLCuMuV, RamV and CoYVVNCP
4.3 Results and Discussion
4.3.1 Rice stripe virus Co-infect Nicotiana benthamina with Cotton leaf curl Multan virus（CLCuMuV）, Ramie mosaic virus （RamV）, Corchorus yellow vein Vietnam virus（CoYVV）
4.3.2 RSV snatches capped RNA leaders from CLCuMuV, RamV and CoYVVmRNAs
Chapter 5 General Discussion
Chapter 6 Conclusions and Future perspective of this study
6.1 Conclusion
6.2 Future Perspective
References
Published Papers and other Academic Achievements during the Study
Acknowledgement
Appendices
Appendix A
Appendix B
【參考文獻】：
期刊論文
[1]傳播木爾坦棉花曲葉病毒的煙粉虱隱種鑒定[J]. 陳婷,湯亞飛,趙蕊,何自福,呂利華. 植物保護學報. 2016(01)
[2]海南紅麻曲葉病的病原檢測與鑒定[J]. 湯亞飛,何自福,杜振國,佘小漫,藍國兵. 植物病理學報. 2015(06)
[3]侵染垂花懸鈴花的木爾坦棉花曲葉病毒分子特征研究[J]. 湯亞飛,何自福,杜振國,韓利芳,佘小漫,羅方芳. 植物病理學報. 2013(02)
[4]福州市發(fā)生由木爾坦棉花曲葉病毒引起的朱槿曲葉病[J]. 章松柏,夏宣喜,張潔,張友軍,吳祖建. 植物保護. 2013(02)
[5]侵染廣東黃秋葵的木爾坦棉花曲葉病毒及伴隨衛(wèi)星DNA的分子特征[J]. 董迪,朱艷華,何自福,柴兆祥,佘小漫,羅方芳. 華南農業(yè)大學學報. 2012(01)
[6]北京地區(qū)番茄黃化曲葉病毒病的鑒定及防治對策[J]. 周濤,師迎春,陳笑瑜,范在豐. 植物保護. 2010(02)
[7]江蘇省番茄黃化曲葉病的病原分子診斷[J]. 季英華,熊如意,程兆榜,周彤,趙統(tǒng)敏,余文貴,范永堅,周益軍. 園藝學報. 2008(12)
[8]侵染朱槿的木爾坦棉花曲葉病毒及其衛(wèi)星DNA全基因組結構特征[J]. 毛明杰,何自福,虞皓,李華平. 病毒學報. 2008(01)
[9]鯉腸黃脈病樣中煙粉虱傳雙生病毒的檢測[J]. 何自福,虞皓,毛明杰,佘小漫. 西北農林科技大學學報(自然科學版). 2005(S1)
[10]廣東番茄曲葉病毒G3分離物基因組DNA-A的分子特征[J]. 何自福,虞皓,羅方芳. 植物病理學報. 2005(03)
本文編號：2904428
【文章來源】：福建農林大學福建省
【文章頁數】：115 頁
【學位級別】：博士
【文章目錄】：
List of Abbreviations
摘要
Abstract
Chapter 1 Introduction and Review of literature
1.1 Geminiviruses: General description
1.2 Geminiviruses:Txonmy
1.3 Geminiviruses: Genome organization
1.4 Geminiviruses: gene function and host-virus interaction
1.5 Geminiviruses: Ecology
1.6 Geminiviruses in China and Fujian Province
1.7 Objectives of Research
Chapter 2 Detection of Begomoviruses from different host plants in Fujian Province ofChina
2.1 Background
2.2 Materials and methods
2.2.1 Collection of samples
2.2.2 Genomic DNA extraction
2.2.3 PCR amplification, cloning, and sequencing
2.2.4 PCR/RCA amplification of DNA P
2.2.5 Phylogenetic analysis
2.3 Results and Discussion
2.3.1 Identification of Begomoviruses and Satellites in Fujian Province of China
2.3.1.1 Detection of Cotton leaf curl Multan virus and Betasatellite from Hibiscussabdariffa
2.3.1.2 Detection of Ramie mosaic virus （RamV） from Ramie （Boehmeria nivea）
2.3.1.3 Detection of Tomato yellow leaf curl virus （TYLCV） from Tomato plants（Lycopersicon esculentum L）
2.3.1.4 Detection of Corchorus yellow vein Vietnam virus （CoYVV） from Jute plants（Corchorus olitorius L.）
2.3.1.5 Detection of Ageratum yellow vein China virus （AYVCnV） infecting Ageratum（Ageratum conyzoides）
2.3.1.6 Detection of Kudzu mosaic virus virus （KuMV） infecting Kudzu （Ageratumconyzoides）
Chapter 3 Construction of Infectious clone and Molecular studies/characterization ofCLCuMuV, RamV and CoYVV
3.1 Background
3.2 Material and Methods
3.2.1 Collection of samples and DNA extraction
3.2.2 Construction of infectious clone and agrobacterium infilteration
3.2.3 Confirmation of infection by Southern Blot analysis
3.2.4 Cloning and sequence analysis:
3.3 Results and Discussion
Chapter 4 Molecular characterization and RSV Co-infect Nicotiana benthamiana withbegomoviruses
4.1 Background
4.2 Material and Methods
4.2.1 Virus Source and inoculation
4.2.2 Detection of TSSs by Southern Blot analysis
4.2.3 Primer Design
4.2.4 High Throughput sequencing of 5' ends of CLCuMuV, RamV and CoYVVNCP
4.3 Results and Discussion
4.3.1 Rice stripe virus Co-infect Nicotiana benthamina with Cotton leaf curl Multan virus（CLCuMuV）, Ramie mosaic virus （RamV）, Corchorus yellow vein Vietnam virus（CoYVV）
4.3.2 RSV snatches capped RNA leaders from CLCuMuV, RamV and CoYVVmRNAs
Chapter 5 General Discussion
Chapter 6 Conclusions and Future perspective of this study
6.1 Conclusion
6.2 Future Perspective
References
Published Papers and other Academic Achievements during the Study
Acknowledgement
Appendices
Appendix A
Appendix B
【參考文獻】：
期刊論文
[1]傳播木爾坦棉花曲葉病毒的煙粉虱隱種鑒定[J]. 陳婷,湯亞飛,趙蕊,何自福,呂利華. 植物保護學報. 2016(01)
[2]海南紅麻曲葉病的病原檢測與鑒定[J]. 湯亞飛,何自福,杜振國,佘小漫,藍國兵. 植物病理學報. 2015(06)
[3]侵染垂花懸鈴花的木爾坦棉花曲葉病毒分子特征研究[J]. 湯亞飛,何自福,杜振國,韓利芳,佘小漫,羅方芳. 植物病理學報. 2013(02)
[4]福州市發(fā)生由木爾坦棉花曲葉病毒引起的朱槿曲葉病[J]. 章松柏,夏宣喜,張潔,張友軍,吳祖建. 植物保護. 2013(02)
[5]侵染廣東黃秋葵的木爾坦棉花曲葉病毒及伴隨衛(wèi)星DNA的分子特征[J]. 董迪,朱艷華,何自福,柴兆祥,佘小漫,羅方芳. 華南農業(yè)大學學報. 2012(01)
[6]北京地區(qū)番茄黃化曲葉病毒病的鑒定及防治對策[J]. 周濤,師迎春,陳笑瑜,范在豐. 植物保護. 2010(02)
[7]江蘇省番茄黃化曲葉病的病原分子診斷[J]. 季英華,熊如意,程兆榜,周彤,趙統(tǒng)敏,余文貴,范永堅,周益軍. 園藝學報. 2008(12)
[8]侵染朱槿的木爾坦棉花曲葉病毒及其衛(wèi)星DNA全基因組結構特征[J]. 毛明杰,何自福,虞皓,李華平. 病毒學報. 2008(01)
[9]鯉腸黃脈病樣中煙粉虱傳雙生病毒的檢測[J]. 何自福,虞皓,毛明杰,佘小漫. 西北農林科技大學學報(自然科學版). 2005(S1)
[10]廣東番茄曲葉病毒G3分離物基因組DNA-A的分子特征[J]. 何自福,虞皓,羅方芳. 植物病理學報. 2005(03)
本文編號：2904428
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