Caspase-7在大鼠顱腦損傷后表達(dá)的免疫組織化學(xué)研究
本文選題:法醫(yī)學(xué) 切入點(diǎn):caspase-7 出處:《重慶醫(yī)科大學(xué)》2006年碩士論文 論文類型:學(xué)位論文
【摘要】: 目的:創(chuàng)傷性顱腦損傷是法醫(yī)工作中經(jīng)常見到的一種損傷,腦損傷后引起的神經(jīng)細(xì)胞死亡通過壞死和凋亡兩種途徑實(shí)現(xiàn)。凋亡發(fā)生是一個(gè)復(fù)雜的,由caspase家族成員介導(dǎo)的蛋白酶級聯(lián)反應(yīng)過程。Caspase-7是caspase級聯(lián)反應(yīng)中的下游分子之一,為由303個(gè)氨基酸組成的蛋白質(zhì),分子量為35kD,存在于多種細(xì)胞系,彌散分布在胞漿內(nèi)和近膜結(jié)構(gòu)上,是凋亡的最終效應(yīng)分子之一。正常情況下Caspase-7以酶原的形式存在,在凋亡過程中被其上游分子活化裂解為兩個(gè)活性亞單位,作用于底物,使凋亡得以執(zhí)行。早期認(rèn)為Caspase-7在中樞神經(jīng)系統(tǒng)凋亡中并無重要作用,但近年的研究提示其似乎在神經(jīng)細(xì)胞的凋亡中扮演重要的角色,故其日益受到關(guān)注。本文采用自由落體撞擊模型和SABC法研究大鼠顱腦損傷后caspase-7的IHC表達(dá)情況,旨在發(fā)現(xiàn)其表達(dá)是否具有規(guī)律性,希望能為法醫(yī)學(xué)顱腦損傷的損傷時(shí)間推斷補(bǔ)充新的指標(biāo)。 方法:采用完全隨機(jī)分組方法,根據(jù)造創(chuàng)后處死時(shí)間不同將實(shí)驗(yàn)大鼠隨機(jī)分為傷后1h、3h、6h、12h、1d、3d、5d、7d、10d、14d組,另設(shè)正常對照組及假手術(shù)組。在預(yù)定時(shí)間分別處死大鼠,取損傷處及其周圍區(qū)域(對照組、假手術(shù)組取與實(shí)驗(yàn)組相同的部位),進(jìn)行HE染色和caspase-7免疫組化SABC法染色,并對染色結(jié)果進(jìn)行光鏡觀察、圖像分析及統(tǒng)計(jì)學(xué)處理,以了解caspase-7在大鼠顱腦損傷后的表達(dá)情況和規(guī)律。
[Abstract]:Objective: traumatic craniocerebral injury is a kind of injury that is often seen in forensic medicine. The death of nerve cells caused by brain injury is realized by two ways: necrosis and apoptosis. The protease cascade process mediated by members of the caspase family. Caspase-7 is one of the downstream molecules in the caspase cascade reaction. It is a 303-amino acid protein with a molecular weight of 35kD.It exists in a variety of cell lines and distributes in the cytoplasm and near membrane structure. Caspase-7 is one of the final effector molecules of apoptosis. Normally, Caspase-7 exists in the form of prozyme and is activated into two active subunits by upstream molecules during apoptosis, acting on the substrate. It is believed that Caspase-7 does not play an important role in central nervous system apoptosis, but recent studies suggest that Caspase-7 may play an important role in neuronal apoptosis. Therefore, it has attracted more and more attention. In this paper, the expression of caspase-7 IHC in rats after craniocerebral injury was studied by free fall impact model and SABC method, in order to find out whether the expression of caspase-7 was regular or not. It is hoped that it can be used to infer the injury time of forensic craniocerebral injury. Methods: the experimental rats were randomly divided into two groups according to the time of death after injury: the rats were randomly divided into three groups: 1 hour after injury, 3 hours after injury, 1 hour after injury, 3 days after injury, 3 days after injury, 5 days after injury, 7 days after 7 days and 10 days after 14 days, and then set up normal control group and sham operation group, and the rats were killed at the scheduled time, and the rats were divided into two groups: the control group and the sham operation group. The site of injury and its surrounding area (control group, sham-operation group and experimental group) were taken for HE staining and caspase-7 immunohistochemical SABC staining. The staining results were observed by light microscopy, image analysis and statistical analysis. To understand the expression and regularity of caspase-7 after craniocerebral injury in rats.
【學(xué)位授予單位】:重慶醫(yī)科大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2006
【分類號(hào)】:D919
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