發(fā)布時(shí)間：2018-03-09 07:05

  本文選題：ELOVL6　切入點(diǎn)：秦川牛　出處：《西北農(nóng)林科技大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：超長(zhǎng)鏈脂肪酸延伸酶6是ELOVLs家族中的第6個(gè)成員,有5個(gè)外顯子、4個(gè)內(nèi)含子�！�10嘆6的功能主要表現(xiàn)在促成C1 6飽和與不飽和脂肪酸轉(zhuǎn)變成為18C脂肪酸。ELOVLs家族的基因在動(dòng)物機(jī)體內(nèi)的控制和表達(dá)調(diào)控主要表現(xiàn)在脂肪,尤其是超長(zhǎng)鏈。脂肪酸是機(jī)體的重要組成部分,關(guān)乎著動(dòng)物體的生命健康,維持著生命的活性,是新陳代謝的重要組成物。尤其脂肪酸還扮演者信號(hào)分子的角色,參與著體內(nèi)的生命代謝和重要的生物學(xué)過程。脂肪的代謝,合成,影響著牛肉的品質(zhì),如口感,大理石花紋,牛的機(jī)體正常發(fā)育,和牛的體質(zhì)健康,從而也影響著牛的生理健康。肉牛的體尺,體重,胴體重也是影響肉牛產(chǎn)量和品質(zhì)的一個(gè)重要標(biāo)志。所以這次試驗(yàn)選擇了該段與脂肪表達(dá)和調(diào)控相關(guān)的基因現(xiàn)嚴(yán)格選取了日齡相近,生活和詞養(yǎng)環(huán)境相同的秦川牛公牛為試驗(yàn)材料,提取DNA,利用檢測(cè)該段基因的多態(tài)位點(diǎn),分析出不同的基因型,研究其顯隱性和試驗(yàn)用牛測(cè)得性狀的一個(gè)關(guān)聯(lián),研究結(jié)果為:1、利用PCR-RFLP技術(shù),找出了秦川牛說0嘆堪因的三個(gè)突變位點(diǎn),第一個(gè)突變位點(diǎn)g.82409AG位于第六條染色體16458977位置;第二個(gè)突變位點(diǎn)g.82508OA位于第六條染色體16459076位置,第三個(gè)突變位點(diǎn)g.3084AG位于第六條染色體的16379652位置。前兩個(gè)位于編碼區(qū)(第二外顯子),第三個(gè)位于非編碼區(qū)(第一內(nèi)含子)。2、三個(gè)突變位點(diǎn)均為中度多態(tài)性(0.25P0.5)。g.82409AG,g.82508GA處于Hardy-Weinberg極度不平衡狀態(tài);g.3084AG處于Hardy-Weinberg平衡狀態(tài)。3、g.82409AG位點(diǎn)的三個(gè)基因型中A A型和B B型對(duì)秦川牛的體高影響顯著(P=0.043),對(duì)體長(zhǎng),腰角寬,體重,胴體重影響不顯著(P0.05);g.82508GA對(duì)秦川牛的體高,體長(zhǎng),腰角寬的有不同程度的影響,且差異顯著(P0.0i),對(duì)體重和胴體重影響不顯著(p0.05);g.3084AG對(duì)秦川牛的體高,體長(zhǎng),腰角寬影響不顯著(P0.05),對(duì)體重和胴體重影響顯著(P0.05)。三個(gè)位點(diǎn)產(chǎn)生的不同基因型對(duì)牛的肌肉,脂肪組織,或者骨骼的發(fā)育有著顯著影響,可以試著作為分子輔助選擇標(biāo)記的一個(gè)位點(diǎn),用來研究秦川牛肉品質(zhì)改善和體型發(fā)育。
[Abstract]:VLFAE 6 is the sixth member of the ELOVLs family. There are 5 exons and 4 introns. 10 the function of sigh 6 is mainly manifested in the control and expression regulation of the genes of the C 16 saturated and unsaturated fatty acids family into 18C fatty acids. Fatty acids, in particular, are important components of the body, vital to the life and health of animals, vital to life, and an important component of metabolism. In particular, fatty acids also play the role of signaling molecules. The metabolism and synthesis of fat affect the quality of beef, such as taste, marbling, the normal development of cattle, and the health of cattle. This also affects the physical health of cattle. The body size, body weight of beef cattle, Carcass weight is also an important indicator of beef yield and quality. Qinchuan cattle bull, which has the same living and breeding environment, was used as experimental material to extract DNA. By detecting the polymorphic loci of this gene, different genotypes were analyzed, and an association between the obvious recessive traits and the traits measured by the test cattle was studied. The result of the study was: 1. Using PCR-RFLP technique, we found three mutation sites in Qinchuan cattle. The first mutation locus (g.82409 AG) was located at 16458977 positions on 6th chromosomes, and the second mutation locus (g.82508OA) was located at 16459076 locus of 6th chromosomes. The third mutation locus, g.3084AG, is located at 16379652 of 6th chromosomes. The first two are located in the coding region (exon 2) and the third in the non-coding region (intron 1. 2). The three mutation sites are all moderately polymorphic, 0.25P0.5, g.82409AGG. 82508GA is located at the Hardy-Weinberg pole. In the three genotypes of Hardy-Weinberg equilibrium, A and B have significant effects on body height and body length of Qinchuan cattle. The waist width, body weight and carcass weight had no significant effects on the body height, body length and waist angle width of Qinchuan cattle, and the difference was significant (P 0. 0. 0), but had no significant effect on body weight and carcass weight of Qinchuan cattle. The influence of waist angle width on body weight and carcass weight was not significant. The different genotypes produced at three loci had significant effects on the development of muscle, adipose tissue, or bone of cattle. It can be used as a site of molecular-assisted selection marker to study the improvement of beef quality and the development of body shape in Qinchuan beef.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S823.92

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 尚友國(guó),宋美玲,王建民;山東地方綿羊MHC-DRB3基因PCR-RFLPs反應(yīng)體系的優(yōu)化[J];草食家畜;2005年02期

2 劉云芳,剡根強(qiáng),王新峰;RFLP技術(shù)在動(dòng)物遺傳育種中的應(yīng)用[J];內(nèi)蒙古畜牧科學(xué);2002年02期

3 武彬;;家畜育種中生化遺傳標(biāo)記的應(yīng)用[J];畜牧獸醫(yī)雜志;1989年03期

4 王威;仇玉蘭;孫品;夏昭林;;幾種常規(guī)實(shí)驗(yàn)室適用的單核苷酸多態(tài)性檢測(cè)方法原理與應(yīng)用[J];職業(yè)衛(wèi)生與應(yīng)急救援;2006年04期

，

本文編號(hào)：1587471