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低蛋白日糧平衡氨基酸對生長豬生長性能及氮排放的影響研究

發(fā)布時間:2019-07-07 07:35
【摘要】:本論文通過飼喂補充必需氨基酸的低蛋白日糧來研究其對生長豬生長性能、N平衡及營養(yǎng)物質表觀消化率的影響。試驗一:選擇60日齡左右、平均體重23.5±1.38kg的三元雜交(杜×長×大)豬192頭,按日齡、體重、性別基本一致的原則隨機分為4組,每組6欄,每欄8頭,試驗期為35d。試驗采用玉米、豆粕及DDGS為主要原料,參考NRC(2012)以及理想氨基酸模型,應用標準回腸可消化氨基酸及凈能體系配制日糧。生長豬日糧CP水平依次為17%、16%、15%和14%,平衡Lys、Met、Thr、Trp、Val等必需氨基酸。試驗以17%蛋白水平日糧為對照組,其余各組為試驗組。消化試驗以三氧化二鉻(Cr2O3)為指示劑,以欄為單位進行糞樣采集。本試驗的主要結果如下:1.飼喂低蛋白氨基酸平衡日糧的生長豬相比于對照組其生長性能沒有顯著的變化(p0.05)。補充Lys、Met、Thr、Trp、Val等必需氨基酸的前提下,日糧CP水平降低到14%不影響生長豬的生長性能。2.低蛋白氨基酸平衡日糧能夠降低糞N和糞P的含量,提高N、P、Ca的表觀消化率。日糧CP水平降低到14%和15%時,糞中N含量分別較CP17%組降低了7.4%和6.2%;CP14%、CP15%和CP16%的日糧糞中P的含量分別比CP17%組下降了1.5%、6.0%、3.8%,且CP15%與CP17%之間差異顯著(p0.05);飼喂CP15%的日糧P的表觀消化率顯著提高(p0.05);CP14%和CP16%的日糧Ca的表觀消化率明顯高于CP17%組,較CP17%組分別提高6.4%和4.7%(p0.05),CP15%的日糧Ca的表觀消化率高于CP17%組,但與CP17%組無顯著性差異(p0.05)。3.低蛋白氨基酸平衡日糧能夠明顯提高EE、CF、DM和Ash的表觀消化率。CP14%和CP 15%的日糧糞中EE含量較CP17%組分別下降了22.4%和20.2%,EE表觀消化率均與CP17%組存在極顯著差異(p0.01);CP14%和CP 15%的日糧CF表觀消化率最高,且與CP17%組間有極顯著差異(p0.01),分別較CP17%組高17.4%和16.7%;DM的表觀消化率隨日糧粗蛋白水平的降低而升高,其中CP14%的日糧DM表觀消化率最高CP15%和CP16%的日糧次之,且均與CP17%組有極顯著差異(p0.01);CP14%和CP15%的日糧糞中Ash含量極顯著低于CP17%組(p0.01),且兩組Ash的表觀消化率最高,與CP17%組間存在極顯著差異(p0.01),分別較CP17%組提高了28.5%和31.0%,CP 16%的日糧與CP17%組間也存在極顯著差異(p0.01)。試驗二:試驗選擇50日齡、體重相近、健康狀況良好、遺傳背景相近的長大二元雜交閹公豬28頭,隨機分組,按體重、遺傳背景基本一致的原則將試驗動物分為4個處理組,每組7個重復,每個重復1頭生長豬。試驗日糧與試驗設計同試驗一。從試驗第15d開始進行為期5d的N平衡試驗,用全收糞尿法收集糞尿。結果表明:降低日糧粗蛋白水平并平衡氨基酸能夠極顯著降低尿N和總N的排放量(p0.01);降低日糧粗蛋白水平使N的表觀生物學價值顯著提高(p0.05);攝入N總量隨著蛋白質水平的降低而降低(p0.05),但各組沉積N量、沉積N/攝入N以及N的糞排泄率差異不顯著(p0.05);日糧蛋白質水平不影響N表觀消化率(p0.05)。
文內圖片:低蛋白日糧平衡氨基酸對營養(yǎng)物質表觀消化率的影響
圖片說明:低蛋白日糧平衡氨基酸對營養(yǎng)物質表觀消化率的影響
[Abstract]:In this paper, the effects of low protein diet supplemented with essential amino acids on growth performance, N balance and apparent digestibility of nutrients in growing pigs were studied. Experiment 1: 192 ternary crossbred pigs (du 脳 long 脳 large) with an average weight of 23.5 鹵1.38kg were randomly divided into 4 groups according to the principle of age, body weight and sex, with 6 columns in each group and 8 pigs in each column. The experimental period was 35 days. Corn, soybean meal and DDGS were used as main raw materials, NRC (2012) and ideal amino acid model were used to prepare diet with standard ileal digestible amino acid and net energy system. The dietary CP levels of growing pigs were 17%, 16%, 15% and 14%, respectively. the essential amino acids such as Lys,Met,Thr,Trp,Val were balanced. 17% protein level diet was used as the control group and the other groups as the experimental group. Chromium trioxide (Cr2O3) was used as indicator and fecal samples were collected in column. The main results of this experiment are as follows: 1. There was no significant change in growth performance between the growing pigs fed with low protein amino acid balanced diet and the control group (p0.05). On the premise of supplementation of essential amino acids such as Lys,Met,Thr,Trp,Val, the decrease of dietary CP level to 14% did not affect the growth performance of growing pigs. 2. Low protein amino acid balanced diet could reduce the content of fecal N and P, and increase the apparent digestibility of Ca. When the dietary CP level decreased to 14% and 15%, the N content in feces decreased by 7.4% and 6.2% when compared with the CP17% group, and the P content in the diet feces of CP15% and CP16% decreased by 1.5%, 6.0% and 3.8%, respectively, and there was a significant difference between CP15% and CP17% (p0.05), and the apparent digestibility of dietary P fed with CP15% was significantly increased (p0.05). The apparent digestibility of dietary Ca in CP14% and CP16% group was significantly higher than that in CP17% group, which was 6.4% and 4.7% higher than that in CP17% group (p0.05). The apparent digestibility of Ca in CP15% group was higher than that in CP17% group, but there was no significant difference between CP15% group and CP17% group (p0.05). The apparent digestibility of EE,CF,DM and Ash was significantly increased by low protein amino acid balanced diet. The content of EE in diet feces of CP14% and CP 15% was 22.4% and 20.2% lower than that of CP17% group, respectively, and the apparent digestibility of EE was significantly different from that of CP17% group (p0.01). The apparent digestibility of dietary CF of CP14% and CP15% was the highest, which was significantly different from that of CP17% group (p0.01). The apparent digestibility of DM was 17.4% and 16.7% higher than that of CP17% group, respectively, with the decrease of dietary crude protein level, and the apparent digestibility of DM in CP14% group was the highest, and that of CP15% and CP16% group was the second, and there was significant difference between CP14% group and CP17% group (p0.01). The content of Ash in diet feces of CP14% and CP15% was significantly lower than that of CP17% group (p0.01), and the apparent digestibility of Ash in the two groups was the highest, which was significantly different from that in CP17% group (p0.01), which was 28.5% and 31.0% higher than that in CP17% group, and there was also significant difference between CP 16% diet and CP17% group (p0.01). Experiment 2: 28 long binary hybrid castrated boars aged 50 days, with similar body weight, good health and similar genetic background, were randomly divided into 4 treatment groups with 7 repeats in each group and 1 growing pig in each group according to the principle that the body weight and genetic background were basically the same. The experimental diet is the same as the experimental design. The N balance test was carried out for 5 days from the 15th day of the experiment, and the feces and urine were collected by the method of collecting feces and urine. The results showed that reducing dietary crude protein level and balancing amino acids could significantly reduce urinary N and total N emissions (p0.01), decrease dietary crude protein level significantly increased the apparent biological value of N (p0.05), and the total N intake decreased with the decrease of protein level (p0.05), but there was no significant difference in N deposition, N / N uptake and fecal excretion rate in each group (p0.05). Dietary protein level did not affect the apparent digestibility of N (p0.05).
【學位授予單位】:華中農業(yè)大學
【學位級別】:碩士
【學位授予年份】:2015
【分類號】:S828.5

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