發(fā)布時(shí)間：2018-02-24 10:28

  本文關(guān)鍵詞： 抗人類原肌球蛋白亞型5（hTM5） 抗中性粒細(xì)胞胞漿抗體（ANCA） 潰瘍性結(jié)腸炎（UC）　出處：《山西醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 通過檢測(cè)UC患者血清hTM5、ANCA與腸粘膜hTM5的表達(dá)，探討hTM5在UC中的臨床意義。 材料和方法 收集2012年4月至2013年6月山西醫(yī)科大學(xué)第一臨床醫(yī)院消化科門診和住院UC患者60例的血清、腸粘膜標(biāo)本及臨床資料，其中UC中重度組30例，UC輕度組30例，診斷符合2012廣州會(huì)議“對(duì)我國(guó)IBD診斷治療規(guī)范的共識(shí)意見”[1]。采用ELISA方法檢測(cè)外周血中原肌球蛋白亞型5抗體含量，對(duì)照組為健康志愿者30例。結(jié)腸粘膜組織行免疫組織化學(xué)方法檢測(cè)腸粘膜原肌球蛋白亞型5陽(yáng)性細(xì)胞數(shù)，對(duì)照組來自結(jié)腸癌行外科手術(shù)切除的正常結(jié)腸斷端。此外,還應(yīng)用間接免疫熒光法檢測(cè)UC患者60例及健康對(duì)照組30例的血清中抗中性粒細(xì)胞胞漿抗體的陽(yáng)性率。 結(jié)果 （1）免疫組織化學(xué)法檢測(cè)結(jié)果：中重度UC患者、輕度UC患者、健康對(duì)照人群結(jié)腸上皮細(xì)胞中原肌球蛋白亞型5陽(yáng)性表達(dá)率差異有統(tǒng)計(jì)學(xué)意義(P＜0.05)。（2）酶聯(lián)免疫吸附法檢測(cè)結(jié)果：中重度UC患者、輕度UC患者、健康對(duì)照人群血清中原肌球蛋白亞型5抗體濃度差異有統(tǒng)計(jì)學(xué)意義(P＜0.05)。（3）間接免疫熒光法檢測(cè)結(jié)果：UC患者血清ANCA陽(yáng)性率明顯高于健康對(duì)照組，差異有統(tǒng)計(jì)學(xué)意義（p＜0.05）。（4）血清hTM5濃度和ANCA陽(yáng)性率做Spearman等級(jí)相關(guān)分析，發(fā)現(xiàn)血清hTM5濃度與ANCA陽(yáng)性率呈正相關(guān)（r=0.428.p=0.000）。 結(jié)論 （1）UC患者結(jié)腸粘膜及血清中原肌球蛋白亞型5抗體的表達(dá)程度與UC病情輕重程度均呈正相關(guān)，提示原肌球蛋白亞型5影響UC的發(fā)病及嚴(yán)重程度。 （2）聯(lián)合檢測(cè)血清原肌球蛋白亞型5和抗中性粒細(xì)胞胞漿抗體有助于對(duì)UC的診斷與嚴(yán)重程度的判斷。
[Abstract]:Purpose. To investigate the clinical significance of hTM5 in UC by detecting the expression of hTM5 ANCA and intestinal mucosal hTM5 in patients with UC. Materials and methods. From April 2012 to June 2013, 60 patients with UC were collected from the Department of Digestive Medicine of the first Clinical Hospital of Shanxi Medical University, including 30 cases of moderate and severe UC and 30 cases of mild UC. The diagnosis was in accordance with the "consensus opinion on the criteria for diagnosis and treatment of IBD in China" [1]. ELISA method was used to detect the level of proto-myosin subtype 5 antibody in peripheral blood. The number of positive cells of myosin subtype 5 in intestinal mucosa was detected by immunohistochemical method in 30 healthy volunteers, and the control group came from the normal cut end of colon cancer surgically resected. The positive rates of anti-neutrophil cytoplasmic antibodies in 60 UC patients and 30 healthy controls were also detected by indirect immunofluorescence assay. Results. Immunohistochemical results: moderate and severe UC patients, mild UC patients, The positive rate of myosin subtype 5 in colonic epithelial cells of healthy controls was significantly different (P < 0.05). The results of enzyme-linked immunosorbent assay (Elisa) were as follows: moderate and severe UC patients, mild UC patients, and mild UC patients. The results of indirect immunofluorescence assay showed that the positive rate of serum ANCA in patients with 20% UC was significantly higher than that in healthy controls. The difference was statistically significant (P < 0.05) the serum hTM5 concentration and the positive rate of ANCA were analyzed by Spearman grade correlation analysis. It was found that the serum hTM5 concentration was positively correlated with the positive rate of ANCA. Conclusion. The expression of myosin subtype 5 antibody in colonic mucosa and serum was positively correlated with the severity of UC, suggesting that myosin subtype 5 affected the incidence and severity of UC. (2) combined detection of promyosin subtype 5 and anti-neutrophil cytoplasmic antibody is helpful to the diagnosis and severity of UC.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R574.62

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本文編號(hào)：1529896