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shMDR1cṲdǼ{˷[ˎо

l(f)r(sh)g2020-11-19 17:35
ĿČmi(Cervical cancer)Ŀǰ(gu)(ni)DҊĐ[,(yn)ӰŮԽ^(qing)D(zhun)ԺA(y)(f)l(f)ʸߵc(din)ĿǰR϶ȡˎί鱣د,SίL(zhng),O׮a(chn)ˎ,ҳˎ֏(f)ˎԵķ,Ķ(du)Ч@ȞҪmi(x)P-ǵ(P-gp)_(d)ˮƽh(yun)(x),w(ni)P-gpߵČmi߸׮a(chn)ˎ@pP-ǵ׵^ȱ_(d)ܳɞ˷miˎP(gun)IMDR1P-gpľa,˰oĬMDR1ЧpP-gp_(d)(Gefitinib),һNƤL(zhng)wҰἤø(EGFR-TK)Ƅ,R(jng)ίС(x),S(du)|(zh)ˎC(j)Ƶо,F(xin)ڼҲV(yng)ڌmiί,ˎF(xin)Įa(chn)(yn)˼Ŀ[˱n}MСɔ_RNAshMDR1(du)ˎM(jn)oĬ,pP-gpı_(d),ˎİl(f),ʹͼmiHela(x)«@Ì(du)ԡоO(sh)Ӌ(j)Ƃ䚤Ǽ{ԭˎcshMDR1ͬڼ{,(sh)F(xin)Ϳ[ˎĹf칲d{ķ(wn)Լ,d{(du)ˎ(x)ֳӰ,̽(x)(du)shMDR1ͼṲd{zȡC(j)ơCǰdcshMDR1п˷[ˎĝ|(zh),ӻЧ(sh)(yn)չȷĺy(c),(du)ľ؏(f)ԡ(wn)ԺͻʵM(jn)п졣xӽ“(lin)cshMDR1dڲϚ,ƂɚǼ{(du)ƂĹd{M(jn)б,^ΑB(ti),ͬr(sh)(du){wጷŶȼL(zhng)ڷ(wn)M(jn)пͨ^zѪ屣o(h)(sh)(yn)ø⌍(sh)(yn)욤nj(du)|(zh)shMDR1ıo(h)á⹲۽@΢R^shMDR1cṲd{ڼ(x)(ni)ķֲrͨ^zȡ(sh)(yn)(x)zȡd{ʽ(du)d{w⿹[M(jn)п졣\(yn)MTT̽shMDR1cṲd{(du)mi(x)ֳӰʽ(x)g(sh)shMDR1cṲd{(du)(x)ӰWestern blot(sh)(yn)zy(c)d{(du)(x)P(gun)׵ı_(d)rY(ji)ļԭˎshMDR1y(c)ԡܶ؏(f)ԡ(wn)ԺͻʾϷW(xu)Ҫ,ʜy(c)ڼcshMDR1w⺬y(c)YxƂshMDR1cṲd{̎,Ƃļ{С,͸R^{ΑB(ti)ʾo,ɢáʽY(ji)@ʾx̎܉ܺõČ߰ڼ{ʞ89.8%7.1%,shMDR188.3%7.2%dǼ{ጷųʬF(xin)pHه,pH5.8l¼Ѹጷ,pH7.4lጷž,shMDR1ڼ{еጷОcț]@,48 hr(sh)shMDR1ڃɷNpHlµӋ(j)ጷʾs75%,wF(xin)߾܏ļ{кܺõጷŵ_Һ⹲۽@΢R^쵽dǼ{܉򸻼(x)܇,ҸОʕr(sh)g̈́هzȡ(sh)(yn)?zi)܉C(x)(du)d{ĔzȡcW(wng)񵰰׽錧(do)P(gun),ͨ^NƄʾ{ڼ(x)(ni)D(zhun)\(yn)هԡMTTcʽY(ji)@ʾshMDR1cṲd{܉ƌmi(x)ֳ,^(qing)ļ(x),܉T(do)(x)@ӵWestern blot(sh)(yn)Y(ji)dǼ{@Ӽ(x)Cleaved caspase-3ı_(d),ͬr(sh)f͵shMDR1܉pM(fi)DR1ĺ,Cd{܉D(zhun)MDR1錧(do)ˎC(j)ơY(ji)ՓоɹƂ˹dshMDR1cĚǼ{,ƂĹd{дС,ΑB(ti),ɢ,pHهጷŵc(din)鱾ĵļ{dw܉Чo(h)|(zh)cˎѪøڭh(hun)±ⱻd{(du)ͼHela(x)@ֳ,C(j)shMDR1M(jn)뼚(x),ˎMDR1ı_(d),(do)P-gp_(d)p,D(zhun)ˎ,ʹˎ(x)Hela֏(f)(du),@ڲh(yun)δ,{׼g(sh)(yng)ڻ򹤳̰l(f)],(chung)ăr(ji)ֵ
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W(xu)λݡ2018
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D,ҊV,D


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D,ҊV,D,


D 1B ҊVD(bio)(zhn)L Y(ji)ʾX SǼ(bio)(zhn)Ʒ CY SLƳɘ(bio)(zhn)õؚw A=0.0212C+0.023

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ų䌦(du)Ậy(c)ĸɔ_D 1A ҊVDD 1B ҊVD2(bio)(zhn)LD 2 Y(ji)ʾX SǼ(bio)(zhn)Ʒ CY S A(j)Ùzy(c)Y(ji)LƳɘ(bio)(zhn)õؚw A=0.0212C+0.023P(gun)ϵ(sh) r0.9991f|(zh) 150 g/mL (ni)P(gun)ϵá
īI(xin)

P(gun)ڿՓ ǰ10l

1 ;;;RJ;;ϩ“(lin)ϼίڷС(x)ΰЧcȫо[J];|t(y)ˎW(xu)W(xu)(bo);201802

2 ɿ;ռ;DZ;﷽A;E;;һڷС(x)ΰ(zhn)ίMeta[J];At(y)W(xu);201801

3 A;;Ȫ;ͮ;;;A;Ƽ;;һNСǼ{dwо[J];ܲ;201712

4 ;L(zhng);;С(x)ΰEGFRί^l(f)ˎC(j)ƵоM(jn)չ[J];Ї(gu)[R;201723

5 sӢ;Ƽ;־;O;־;;;ጦ(du)R~̥l(f)[ˎ_(d)Ӱ[J];ؑct(y)W(xu);201732

6 x;S;SP;ؼ;ܺꞮ;m;;@ˎС(x)ΰ(x)в_(d)h(hun)RNA[J];[;201711

7 Ϫ;;;;;Ƭܳ|(zh)ĺYx[J];Ї(gu)t(y)ˎָ;201724

8 ;ƽ;T;F;;Ƃw(ni)⿼[J];Ї(gu)t(y)ˎI(y)s־;201705

9 A;w;o;÷;;;;;dK{֬|(zh)w(du)ɌmiHeLa(x)ֲw(ni)[J];ϺͨW(xu)W(xu)(bo)(t(y)W(xu));201705

10 ʺ;ꐿ;;ؐο“(lin)ϼƬίС(x)ΰRо[J];Ї(gu)RˎW(xu)s־;201708



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