發(fā)布時間：2018-06-18 19:43

  本文選題：金屬納米顆粒 + 熒光納米簇��； 參考：《哈爾濱師范大學》2015年碩士論文

【摘要】：金屬納米顆粒由于具有獨特的電子結構和良好的物理化學性質,被廣泛應用到生物檢測、生化傳感、光學成像等領域。其中利用金屬納米顆粒表面的等離子體共振性質,將其作為比色探針和熒光探針檢測水環(huán)境和細胞內的重金屬離子已成為研究領域的熱點。但目前利用金屬納米顆粒檢測重金屬離子的研究仍需要進一步發(fā)展。合成具有響應時間短、靈敏度高、生物相容性好、毒性較低等性質的金屬納米顆粒檢測探針是我們今后的研究方向。我們合成了具有熒光性質的金、銀、銅納米簇作為檢測探針檢測重金屬離子,并進行了細胞成像研究,將表面功能化的金納米顆粒作為檢測探針檢測重金屬離子。具體的研究內容如下:(1)硫辛酸功能化金納米顆粒(TA-GNPs)作為比色器件應用于檢測水溶液中的Hg2+。如果水溶液中存在Hg2+,TA-GNPs會發(fā)生聚集導致溶液的顏色發(fā)生變化,達到對水溶液中Hg2+的檢測目的。在本實驗中,對TA-GNPs的靈敏度和選擇性進行測試研究,得出TA-GNPs具有較高的靈敏度和較好的選擇性,TA-GNPs檢測Hg2+的最低檢測限為10 nM。(2)葉酸功能化的銀納米顆粒(FA-AgNPs)作為比色和熒光雙功能探針檢測Hg2+,當溶液中存在Hg2+時,FA-AgNPs會發(fā)生聚集使溶液的顏色發(fā)生變化,并且在275 nm波長的激發(fā)下,FA-AgNPs在440 nm處會有熒光發(fā)射。通過吸光度、熒光強度分別與Hg2+濃度的線性關系得出最低檢測限為1 nM。通過應用性實驗的檢測結果得出FA-AgNPs作為檢測探針有著較好的環(huán)境適應性,能夠準確地檢測出Hg2+是否存在。(3)合成葉酸功能化的熒光金納米簇(FA-Au-NCs),并將FA-Au-NCs作為熒光探針檢測水環(huán)境中與細胞內的Hg2+,同時還進行了細胞成像的研究。所合成的FA-Au-NCs在275 nm處激發(fā)在440 nm處有強的藍光發(fā)射,其熒光壽命為6.11 ns,量子效率為21%。在檢測過程中,水溶液中或細胞內如果存在Hg2+,熒光探針會出現(xiàn)淬滅現(xiàn)象,結合與熒光光譜的測試,使FA-Au-NCs對水溶液中Hg2+的最低檢測限達到1 nM。通過對環(huán)境水體樣品和Hela細胞內的Hg2+進行檢測,證明FA-Au-NCs熒光探針有著很好的實際應用性。(4)葉酸功能化的熒光銅納米簇(FA-Cu-NCs)可以作為熒光檢測探針檢測水溶液與細胞內Hg2+,并進行了細胞成像的研究。合成的FA-Cu-NCs在275 nm處激發(fā)在440 nm處有較強的藍光發(fā)射。在檢測的過程中,FA-Cu-NCs對Hg2+能夠快速準確的進行檢測。通過對FA-Cu-NCs的實際應用性研究證明其具有很好的環(huán)境適應性。
[Abstract]:Metal nanoparticles have been widely used in biological detection, biochemical sensing, optical imaging and other fields because of their unique electronic structure and good physical and chemical properties. Among them, the use of metal nanoparticles surface plasmon resonance, as a colorimetric probe and fluorescence probe detection of heavy metal ions in water environment and cells has become a hot research field. However, the study of metal nanoparticles for the detection of heavy metal ions still needs further development. The preparation of metal nanoparticles detection probes with short response time, high sensitivity, good biocompatibility and low toxicity is our research direction in the future. We synthesized gold, silver and copper nanoclusters with fluorescence properties as detection probes for heavy metal ions, and carried out cell imaging studies. Surface functionalized gold nanoparticles were used as detection probes to detect heavy metal ions. The specific research contents are as follows: (1) the functional gold nanoparticles (TA-GNPs) of lipoic acid are used as colorimetric devices for the detection of Hg _ 2 in aqueous solution. If there is Hg2 + TA-GNPs in aqueous solution, the color of the solution will change due to the aggregation, and the detection of Hg2 in aqueous solution will be achieved. In this experiment, the sensitivity and selectivity of TA-GNPs were studied. The results showed that TA-GNPs have higher sensitivity and better selectivity. The minimum detection limit of TA-GNPs for detection of Hg2 is 10 nM.f2). FA-AgNPsof silver nanoparticles functionalized by folic acid can be used as colorimetric and fluorescent probes for detection of Hg2. When Hg2 exists in the solution, the concentration of FA-AgNPs changes the color of the solution, and the fluorescence emission of FA-AgNPs is observed at 440 nm under the excitation of 275 nm. The linear relationship between fluorescence intensity and Hg2 concentration was obtained by absorbance, and the minimum detection limit was 1 nm. The results of the applied experiments show that FA-AgNPs as a detection probe has good environmental adaptability. It is possible to accurately detect the existence of Hg2) in the synthesis of folic acid functionalized gold nanoclusters FA-Au-NCsN, and use FA-Au-NCs as a fluorescence probe to detect Hg2 in water environment and in cells. At the same time, cell imaging has been carried out. The synthesized FA-Au-NCs exhibit strong blue emission at 275 nm at 440 nm with a fluorescence lifetime of 6.11 ns and a quantum efficiency of 21 nm. In the process of detection, the fluorescence probe will quench if there is Hg _ 2 in aqueous solution or in cells. The minimum detection limit for Hg _ 2 in aqueous solution can reach 1 nm by FA-Au-NCs combined with fluorescence spectrum test. The detection of Hg2 in environmental water samples and Hela cells shows that FA-Au-NCs fluorescent probe has a good practical application. (4) folic acid functionalized fluorescent copper nanoclusters FA-Cu-NsCs) can be used as fluorescent detection probes for detection of Hg2 in aqueous solution and in cells, and the results show that FA-Au-NCs fluorescent probe can be used as a fluorescent probe to detect Hg2 in aqueous solution and in cells. Cell imaging was also studied. The synthesized FA-Cu-NCs have strong blue emission at 440nm excited at 275 nm. In the process of detection, FA-Cu-NCs can detect Hg 2 quickly and accurately. Through the practical application of FA-Cu-NCs, it is proved that FA-Cu-NCs has good environmental adaptability.
【學位授予單位】：哈爾濱師范大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：O657.3;TB383.1

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本文編號：2036648