發(fā)布時間：2018-01-08 10:02

  本文關(guān)鍵詞：N-乙酰半胱氨酸對膿毒癥大鼠腸損傷的保護作用及對炎癥因子表達的影響　出處：《沈陽農(nóng)業(yè)大學(xué)學(xué)報》2017年02期 　論文類型：期刊論文

  更多相關(guān)文章： N-乙酰半胱氨酸 膿毒癥 大鼠 腸粘膜

【摘要】：為探討N-乙酰半胱氨酸(NAC)對膿毒血癥大鼠腸損傷的影響及對炎癥因子表達的影響,選取清潔級SD大鼠30只,體重250~300g,隨機分為假手術(shù)組(Sham組)、膿毒癥組(SEP組)和N-乙酰半胱氨酸組(NAC組),每組10只;Sham組僅開腹,暴露盲腸,NAC組和SEP組行盲腸結(jié)扎穿孔術(shù),再分別靜脈給予150mg·kg~(-1)NAC和等量生理鹽水;3組均于再灌注4h處死大鼠,觀察小腸組織病理學(xué)結(jié)果;ELISA檢測血中腸脂肪酸結(jié)合蛋白(I-FABP)和D-乳酸濃度;ELISA法和Western blot法檢測血清和小腸中TNF-α、IL-6、IL-10濃度。結(jié)果表明:光鏡下Sham組未出現(xiàn)病理損傷,SEP組損傷重,NAC組損傷減輕;與Sham組比較,SEP組I-FABP、D-乳酸顯著升高(p0.05);與SEP組比較,NAC組I-FABP、D-乳酸顯著降低(p0.05);與Sham組比較,SEP組血清中TNF-α、IL-6、IL-10顯著升高(p0.05);與SEP組比較,NAC組TNF-α、IL-6顯著降低(p0.05),IL-10顯著升高(p0.05),小腸炎癥相關(guān)因子變化趨勢同血清檢測。N-乙酰半胱氨酸對膿毒血癥大鼠腸黏膜屏障有保護作用,其機制與抑制炎癥反應(yīng)有關(guān)。
[Abstract]:To investigate the effects of N-acetylcysteine (NACN) on intestinal injury and the expression of inflammatory cytokines in sepsis rats, 30 SD rats of clean grade were selected, weighing 250 ~ 300g. They were randomly divided into sham-sham group, septic group (SEP group) and N-acetylcysteine group (NAC group, n = 10 in each group). In the Sham group, the caecum ligation and perforation were performed in the exposed caecum group and the SEP group, and were given 150 mg 路kg~(-1)NAC and the same amount of normal saline respectively. All the rats in the 3 groups were killed at 4 h after reperfusion, and the histopathological results of small intestine were observed. The concentrations of serum fatty acid binding protein (I-FABP) and D-lactic acid were detected by ELISA. ELISA and Western blot were used to detect the concentration of IL-10 in serum and small intestine. The results showed that there was no pathological injury in Sham group under light microscope. The damage of SEP group was alleviated. Compared with Sham group, I-FABPnD-lactic acid in SEP group was significantly higher than that in Sham group (P 0.05). Compared with SEP group, I-FABP- D- lactic acid in NAC group was significantly lower than that in SEP group. Compared with the Sham group, the serum TNF- 偽 IL-6 + IL-10 in the Sham group was significantly higher than that in the Sham group (P 0.05). Compared with SEP group, TNF- 偽 IL-6 in NAC group decreased significantly (P 0.05) and IL-10 increased significantly (p 0.05). The change of inflammatory related factors in small intestine is related to the protective effect of serum. N-acetylcysteine on intestinal mucosal barrier in rats with sepsis, and its mechanism is related to the inhibition of inflammatory reaction.
【作者單位】： 中國醫(yī)科大學(xué)實驗動物部;
【基金】：國家自然科學(xué)基金項目(81473625)
【分類號】：R459.7
【正文快照】： 膿毒癥(sepsis)是臨床常見的危急重癥之一,許多疾病病理生理學(xué)改變都會引起膿毒血癥,其本質(zhì)是機體由于感染等因素致使大量炎癥因子釋放,引發(fā)全身炎癥反應(yīng)綜合癥(systemic inflmmnatory response syndrome,SIRS),導(dǎo)致機體自身免疫性損傷。膿毒癥的發(fā)病機理復(fù)雜,不同的病因其機

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