發(fā)布時(shí)間：2018-03-09 19:40

  本文選題：輸血　切入點(diǎn)：STR基因座　出處：《四川大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 探討不同輸血量、輸血后不同時(shí)間供者STR_s基因型對受者SWR_s基因型檢測結(jié)果是否有影響。方法3例患者分別接受輸血400ml、800ml及1200ml,輸血前以及輸血后分別在4h、8h、12h采集受者血液,受者和供者血樣用EDTA抗凝,采用Chelex-100法提取DNA,—4℃保存?zhèn)溆谩＿x擇D1S549,D18S865,D3S1754,D12S391,D12S375,D6S477等6個(gè)基因座進(jìn)行PCR擴(kuò)增,電泳分型。結(jié)果3例患者分別輸血400ml、800ml及1200ml后,輸血前及輸血后12h內(nèi)采集血樣檢測的6個(gè)基因座STRs基因型完全一致。結(jié)論 本次研究結(jié)果表明,輸血量在1200ml以下的受者輸血后12h內(nèi)STR_s基因型檢測結(jié)果未受供者血液STR_s基因型的干擾。
[Abstract]:Objective to investigate the effect of STR_s genotypes on the detection of SWR_s genotypes in recipients with different blood transfusions and donor genotypes at different time after transfusion. Methods three patients received 400ml and 1200ml of blood transfusion respectively. The blood samples were collected from the recipients before and after blood transfusion for 8 h and 12 h, respectively. The recipient and donor blood samples were extracted and stored at 4 鈩，

本文編號：1589971