發(fā)布時(shí)間：2019-02-22 12:18

【摘要】：本論文旨在研發(fā)一款針對(duì)體力疲勞有輔助保護(hù)作用的保健食品,研究以人參、鹿胎、玫瑰花、黃精為原料,以白砂糖為輔料制成的保健葡萄酒。對(duì)提取工藝進(jìn)行考察,分別采用滲濾法、熱回流法、冷浸法制備人參鹿胎抗疲勞葡萄酒,以總皂苷的含量以及葡萄酒的感官評(píng)價(jià)為指標(biāo)綜合評(píng)價(jià),篩選出最佳制備方法。以料液質(zhì)量比、提取時(shí)間、提取次數(shù)為因素設(shè)計(jì)正交試驗(yàn),以上述指標(biāo)為依據(jù),優(yōu)選出最佳工藝條件為采用冷浸法,正交實(shí)驗(yàn)最佳條件為料液質(zhì)量比1:10、每次冷浸1天、冷浸2次。對(duì)制劑成型工藝的考察,分別考察離心轉(zhuǎn)速、離心時(shí)間對(duì)樣品產(chǎn)生沉淀的影響,確定最佳的離心條件確定最佳的離心條件為4000 r/min時(shí)間為6 min。多個(gè)樣品中分別加入一定量的白砂糖或蜂蜜,根據(jù)感官評(píng)價(jià)得到最宜加入白砂糖,加入量為3g/100mL。對(duì)產(chǎn)品進(jìn)行質(zhì)量標(biāo)準(zhǔn)研究,考察紫外分光光度法測(cè)定人參鹿胎抗疲勞葡萄酒中總皂苷的含量的精密性、可重復(fù)性和加標(biāo)回收率確定其線(xiàn)性范圍,驗(yàn)證了該方法穩(wěn)定、可行。對(duì)人參鹿胎抗疲勞葡萄酒進(jìn)行功能學(xué)評(píng)價(jià),各劑量組血清尿素氮含量與對(duì)照組比較均有降低,高劑量組與空白組的差別統(tǒng)計(jì)學(xué)意義,能夠提升小鼠肝糖原儲(chǔ)備能力,增加小鼠游泳時(shí)長(zhǎng),高劑量組與空白組差別有統(tǒng)計(jì)學(xué)意義;游泳后曲線(xiàn)下面積雖與對(duì)照組比較無(wú)統(tǒng)計(jì)學(xué)意義,但有下降的趨勢(shì),可見(jiàn)人參鹿胎抗疲勞葡萄酒具有緩解體力疲勞的功效。對(duì)人參鹿胎抗疲勞葡萄酒進(jìn)行毒理學(xué)評(píng)價(jià),急性毒性試驗(yàn)中MTD高于21 g/kg BW,屬于無(wú)毒級(jí);微核試驗(yàn)、Ames試驗(yàn)、精子畸形試驗(yàn)結(jié)果均為陰性;三十天喂養(yǎng)試驗(yàn)每項(xiàng)指標(biāo)都沒(méi)有發(fā)現(xiàn)不良影響。可見(jiàn)人參鹿胎抗疲勞葡萄酒安全可靠。
[Abstract]:The purpose of this paper is to develop a kind of health food which can protect physical fatigue, and study the health care wine made from ginseng, deer fetus, rose and yellow essence, and white sugar as auxiliary material. The extraction process was investigated and the best preparation method was obtained by synthetically evaluating the content of total saponins and the sensory evaluation of the wine by using percolation method heat reflux method and cold soaking method to prepare Panax ginseng deer fetal anti-fatigue wine with the content of total saponins as well as the sensory evaluation of the wine. The orthogonal experiment was designed with the mass ratio of material to liquid, extraction time and times of extraction as the factors. According to the above indexes, the optimum technological conditions were as follows: using cold leaching method. The optimum condition of orthogonal experiment was that the mass ratio of material to liquid was 1: 10, and the cold soaking was 1 day each time. Cold dip twice. The effects of centrifugal speed and time on the precipitation of the sample were investigated. The optimum centrifugation conditions were determined as 4000 r/min and 6 min.. A certain amount of white sugar or honey was added to several samples. According to the sensory evaluation, the most suitable addition of white sugar was 3 g / 100 mL. The quality standard of the product was studied. The precision of the determination of total saponins in ginseng deer fetal antifatigue wine by ultraviolet spectrophotometry was investigated. The repeatability and the recovery rate were determined. The method was proved to be stable and feasible. The functional evaluation of ginseng deer fetal antifatigue wine showed that the serum urea nitrogen content in each dose group was lower than that in the control group, and the difference between the high dose group and the blank group was statistically significant, which could improve the liver glycogen reserve ability of mice. Increase the swimming time of mice, the difference between the high dose group and the blank group was statistically significant; The area under the curve after swimming had no statistical significance compared with the control group, but it had a downward trend. It can be seen that ginseng deer fetal antifatigue wine has the effect of relieving physical fatigue. The toxicological evaluation of ginseng deer fetal antifatigue wine showed that MTD was higher than 21 g/kg BW, in acute toxicity test, but the results of micronucleus test, Ames test and sperm malformation test were all negative. No adverse effects were found in each index of the 30-day feeding trial. It can be seen that ginseng deer fetal anti-fatigue wine is safe and reliable.
【學(xué)位授予單位】：長(zhǎng)春工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：TS262.6

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