發(fā)布時(shí)間：2018-02-26 07:26

  本文關(guān)鍵詞： 葡萄糖 小鼠 -細(xì)胞阻滯 胚胎培養(yǎng) 體外發(fā)育 囊胚率 桑椹胚 體外培養(yǎng) 孵化率 無(wú)糖　出處：《動(dòng)物學(xué)研究》2007年05期 　論文類(lèi)型：期刊論文

【摘要】：研究葡萄糖在小鼠早期胚胎體外發(fā)育中的作用。實(shí)驗(yàn)1將6—8周齡的ICR雌鼠超數(shù)排卵后與公鼠交配,收集1-細(xì)胞放入含0(對(duì)照組)、0.5、1、3、5、10mmol/L葡萄糖的CZB中培養(yǎng);實(shí)驗(yàn)2將從超排的ICR雌鼠輸卵管內(nèi)收集的1-細(xì)胞放入無(wú)糖CZB中培養(yǎng),分別于1細(xì)胞、2細(xì)胞、4細(xì)胞、桑椹胚階段移入含3.0mmol/L葡萄糖(最適濃度)的CZB中,培養(yǎng)24h后又移回到無(wú)糖CZB中(桑椹胚階段除外)繼續(xù)培養(yǎng)以及整個(gè)胚胎培養(yǎng)過(guò)程均在含糖CZB中,對(duì)照組胚胎培養(yǎng)全程均在無(wú)糖CZB中。每組胚胎于37℃、5%CO2培養(yǎng)箱中培養(yǎng)120h,每24h在倒置顯微鏡下觀(guān)察胚胎發(fā)育情況,分別計(jì)算2-細(xì)胞率、4-細(xì)胞率、桑椹胚率、囊胚率和孵化率,并進(jìn)行囊胚細(xì)胞計(jì)數(shù)。結(jié)果顯示,小鼠胚胎在含糖CZB中與在無(wú)糖CZB中4-細(xì)胞發(fā)育率無(wú)差異;含糖CZB中囊胚率顯著高于對(duì)照組;3.0mmol/L濃度組囊胚細(xì)胞數(shù)顯著高于其余組;2-細(xì)胞至4-細(xì)胞、4-細(xì)胞至桑椹胚前添加葡萄糖囊胚率顯著高于對(duì)照組,1-細(xì)胞至2-細(xì)胞、桑椹胚及其以后階段添加葡萄糖囊胚率與對(duì)照組無(wú)差異。實(shí)驗(yàn)證實(shí),在ICR小鼠胚胎體外培養(yǎng)中加入葡萄糖不會(huì)導(dǎo)致2-細(xì)胞阻滯;葡萄糖濃度增至10mmol/L對(duì)ICR小鼠胚胎無(wú)毒性作用;ICR小鼠胚胎體外培養(yǎng)的最適葡萄糖濃度為3.0mmol/L;2-細(xì)胞至4-細(xì)胞、4-細(xì)胞至桑椹胚前添加葡萄糖是必要的。
[Abstract]:To study the role of glucose in the development of mouse early embryos in vitro. In experiment 1, ICR female mice aged 6-8 weeks were superovulated and mated with male mice, and 1- cells were collected and cultured in CZB containing 0 (control group) (control group, 0. 5 ~ 0. 5) and 10 mmol / L glucose. In experiment 2, 1- cells collected from oviducts of superovulation ICR female oviducts were cultured in sugar-free CZB and transferred into CZB containing 3.0 mmol / L glucose (optimal concentration) at morulae stage. After 24 hours of culture, it was transferred back to sugar-free CZB (except morula stage) and the whole process of embryo culture was in sugary CZB. The embryos of the control group were cultured in sugar-free CZB for 120 hours in 37 鈩，

本文編號(hào)：1537050