Protective effects of terminal ileostomy against bacterial t
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AIM:To investigate the effects of terminal ileostomy on bacterial translocation(BT)and systemic inflammation after intestinal ischemia/reperfusion(I/R)injury in rats.METHODS:Thirty-two rats were assigned to either the sham-operated group,I/R group,I/R+resection and anastomosis group,or the I/R+ileostomy group.The superior mesenteric artery was occluded for 60 min.After 4 h,tissue samples were collected for analysis.BT was assessed by bacteriologic cultures,intestinal permeability and serum level...
【文章來源】:World Journal of Gastroenterology. 2014年47期
【文章頁數(shù)】:9 頁
【文章目錄】:
INTRODUCTION
MATERIALS AND METHODS
Animals
Operative procedure
Microbiologic analysis
Wet-to-dry weight ratio
Assay of intestinal permeability
Endotoxin and serum levels of TNF-α, IL-6 and IL-10assays
Assay of myeloperoxidase activity
Histopathology
Statistical analysis
RESULTS
Bacterial translocation
Ileal bacterial counts
W/D weight ratio
Assay of intestinal permeability
Endotoxin assays
Assay of TNF-α, IL-6 and IL-10 in serum
Assay of MPO activity
Histopathology
DISCUSSION
COMMENTS
本文編號:2934655
【文章來源】:World Journal of Gastroenterology. 2014年47期
【文章頁數(shù)】:9 頁
【文章目錄】:
INTRODUCTION
MATERIALS AND METHODS
Animals
Operative procedure
Microbiologic analysis
Wet-to-dry weight ratio
Assay of intestinal permeability
Endotoxin and serum levels of TNF-α, IL-6 and IL-10assays
Assay of myeloperoxidase activity
Histopathology
Statistical analysis
RESULTS
Bacterial translocation
Ileal bacterial counts
W/D weight ratio
Assay of intestinal permeability
Endotoxin assays
Assay of TNF-α, IL-6 and IL-10 in serum
Assay of MPO activity
Histopathology
DISCUSSION
COMMENTS
本文編號:2934655
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