發(fā)布時(shí)間：2018-02-24 05:23

  本文關(guān)鍵詞： B-H Con A-誘導(dǎo)性肝損傷 保護(hù)作用 機(jī)制　出處：《四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2015年06期 　論文類型：期刊論文

【摘要】：目的探討B(tài)7-H4對(duì)免疫性損傷肝臟的保護(hù)作用及其機(jī)制。方法將KM小鼠分為4組(每組15只):生理鹽水組(A組)、pcDNA3.1-mB7-H4-Fc組(B組)、pcDNA3.1組(C組)和刀豆球蛋白A(Con A)組(D組)。B組、C組和D組動(dòng)物均通過(guò)尾靜脈注射Con A 25mg/kg制備肝損傷模型。在Con A注射前1d,分別給予B組每只小鼠注射pcDNA3.1-mB7-H4-Fc 100μg和C組每只小鼠注射pcDNA3.1 100μg。并于注射Con A后12h、24h和48h,各組取5只小鼠,摘眼球取血,分離血清行白介素-4(IL-4)、γ-干擾素(IFN-γ)、谷丙轉(zhuǎn)氨酶(ALT)和谷草轉(zhuǎn)氨酶(AST)濃度的測(cè)定;引頸處死小鼠后取肝臟,用于組織病理學(xué)檢測(cè)。結(jié)果小鼠血清IL-4、IFN-γ、ALT和AST水平在注射Con A后各時(shí)點(diǎn),B、C和D三組較A組均有升高(P0.05),但B組較C組和D組減低,B組與C組間差異有統(tǒng)計(jì)學(xué)意義(P0.01)。小鼠肝組織切片經(jīng)HE染色,光學(xué)顯微鏡下可見(jiàn),B組小鼠肝臟損傷均比C組和D組有不同程度的減輕。結(jié)論初步證實(shí)了B7-H4對(duì)Con A誘導(dǎo)的肝臟免疫性損傷具有保護(hù)作用。B7-H4可能是通過(guò)抑制IL-4和IFN-γ的產(chǎn)生和/或分泌而發(fā)揮對(duì)肝臟保護(hù)的作用。
[Abstract]:Objective to investigate the protective effect of B7-H4 on immune liver injury and its mechanism. Methods km mice were divided into 4 groups (15 rats in each group: pcDNA3.1-mB7-H4-Fc group, pcDNA3.1-mB7-H4-Fc group) and concanavalin A concanavalin A (Con A) group. Rats in group D and group D were injected with Con A 25 mg / kg via tail vein to make liver injury model. Each mouse in group B and group C were injected with pcDNA3.1-mB7-H4-Fc 100 渭 g and pcDNA3.1 100 渭 g 1 day before injection of Con A. after 12 h and 48 h of Con A injection, 5 mice in each group were taken from each group. The serum levels of IL-4, IFN- 緯, alanine aminotransferase (alt) and aspartate aminotransferase (AST) were determined, and the liver was taken after the mice were killed by neck drawing. Results the levels of serum IL-4 IFN- 緯 -alt and AST in mice were higher than those in group A at each time point after Con A injection, but the difference between group B and group C was significantly lower than that in group C and group D. The sections of rat liver were stained with HE. Under optical microscope, the liver injury in group B was significantly less than that in group C and group D. ConclusionThe protective effect of B7-H4 on liver immune injury induced by Con A. B7-H4 may be mediated by inhibiting IL-4 and IFN- 緯. And / or secreted to protect the liver.
【作者單位】： 海南醫(yī)學(xué)院附屬醫(yī)院核醫(yī)學(xué)科;廣東省醫(yī)學(xué)分子診斷重點(diǎn)實(shí)驗(yàn)室;
【基金】：海南省衛(wèi)生廳科研基金項(xiàng)目(瓊衛(wèi)2010-3)資助
【分類號(hào)】：R575

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