西格列汀對肥胖合并非酒精性脂肪肝大鼠的代謝調(diào)節(jié)作用及機(jī)制的探討
[Abstract]:Objective: with the development of social economy and the change of living style, people eat more calories in their daily meals, and the life style of sit-ins is more and more common, which leads to excess energy and fat accumulation in the body. Obesity is a chronic metabolic disease caused by excessive fat accumulation and / or abnormal distribution and weight gain in the body. It is a chronic metabolic disease caused by the interaction of genetic factors, environmental factors and other factors. Overweight and obesity have become one of the severe public health crises in the world. As one of the main components of metabolic syndrome, obesity is closely related to many diseases such as type 2 diabetes, dyslipidemia, hypertension, coronary heart disease, stroke, tumor and so on. Too much fat deposits in the visceral organs, causing the visceral organs, the most common fatty liver, called fatty liver. The important metabolic processes such as coagulation factor and albumin synthesis, toxin degradation and bilirubin circulation in lipopolysaccharide hepatocytes can not be carried out smoothly, which leads to the occurrence and development of a series of metabolic diseases. Therefore, the effect of siglitatin on glucose and lipid metabolism and oxidative stress in obese rats with non-alcoholic fatty liver was studied. Methods: the experimental SD rats were randomly divided into normal diet group (ND group), high-fat diet group, high-calorie high-fat diet group and high-fat diet group for 16 weeks. The rats in high-fat diet group were randomly divided into obese control group (OB group) and siglitatin intervention group (ST group). Normal diet group and obese control group were treated with saline for 6 weeks. Fasting blood glucose (FPG), fasting serum insulin (FINS), serum triglyceride (TG) and serum superoxide dismutase (SOD), serum malondialdehyde (MDA), were measured before and after intervention in each group of experimental rats. The level of serum glutathione peroxidase (GSH-Px) and the levels of GSH-Px,SOD,MDA and TG in 10% liver homogenate after intervention were calculated, and insulin resistance index (HOMA-IR) was calculated. Oil red O staining was used to observe the degree of fatty degeneration in pathological sections of liver. Results: 1). Before intervention, there was no statistical difference between OB group and ST group (P0.05). In OB group, ST group and ND group, the FPG,FINS,TG,MDA level was lower (P0.05) and the serum SOD,GSH-Px level was higher (P0.05). After intervention, the concentrations of TG and MDA in liver tissue of ST group were higher than those in ND group (P0.05), but there was no significant difference in FPG,FINS, serum TG,HOMA-IR, serum MDA, serum and liver tissue SOD,GSH-Px concentration (P0.05). The degree of liver fat variant was increased. Compared with OB group, the concentration of MDA,TG in serum and liver of ST group was decreased, the concentration of GSH-Px,SOD in serum and liver tissue was increased, the difference was statistically significant (P0.05), and the degree of hepatic fatty degeneration was decreased. Conclusion: siglitatin can regulate the metabolism of glucose and lipid in obese rats with non-alcoholic fatty liver and decrease the degree of hepatic fatty degeneration, which may be achieved by regulating the oxidative stress reaction.
【學(xué)位授予單位】:安徽醫(yī)科大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2017
【分類號(hào)】:R575;R589.2
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