細(xì)胞介電泳空間操作及微組裝的自動(dòng)化技術(shù)研究
發(fā)布時(shí)間：2024-05-21 20:07
組織工程學(xué)是一門多學(xué)科交叉的研究領(lǐng)域,其目的是在體外構(gòu)建人造組織,然后將其移植到體內(nèi)進(jìn)行組織的修復(fù)和再生。組織的構(gòu)造通常包括兩個(gè)主要的步驟,首先是從病人的骨髓中取得干細(xì)胞,隨后將這些細(xì)胞在生物相容性的支架上進(jìn)行培養(yǎng)以此來促使組織的發(fā)育。然而大多數(shù)現(xiàn)有的支架結(jié)構(gòu)都過于簡單,難以控制細(xì)胞的分布。本論文中,我們設(shè)計(jì)了新型的生物支架結(jié)合介電泳技術(shù)來實(shí)現(xiàn)細(xì)胞在支架上的自動(dòng)種植。本文由以下三個(gè)方面展開:首先,提出了一種由多層生物相容性材料構(gòu)成的新型生物支架,該支架結(jié)合介電泳技術(shù)實(shí)現(xiàn)了對生物細(xì)胞的三維操作和排列。我們檢驗(yàn)了不同的工藝參數(shù)對三維細(xì)胞構(gòu)型的質(zhì)量和細(xì)胞成活率的影響。實(shí)驗(yàn)結(jié)果表明我們所提出的三維支架能夠利用介電泳力機(jī)理實(shí)現(xiàn)細(xì)胞的自動(dòng)排列,從而構(gòu)建組織工程學(xué)上所需的人造組織。不同的外加電壓所產(chǎn)生的電場力對被操作生物細(xì)胞生存率的影響也做了評估,選擇了最合適的外加電場。其次,設(shè)計(jì)了一種蜂窩狀的模擬原始骨組織結(jié)構(gòu)的多孔生物支架。該支架能夠自動(dòng)將細(xì)胞排列成多層的蜂窩狀構(gòu)型。我們使用三種不同的哺乳動(dòng)物的細(xì)胞進(jìn)行實(shí)驗(yàn),以檢測影響蜂窩狀細(xì)胞構(gòu)型形成過程中的不同因素。我們還對材料的生物相容性進(jìn)行了測試,以保證...
【文章頁數(shù)】：112 頁
【學(xué)位級別】：博士
【文章目錄】：
摘要
ABSTRACT
Chapter 1 Introduction
1.1 Background
1.2 Statement of Problems
1.3 Research Objectives
1.4 Methodology and Significance
1.5 Conclusion
Chapter 2 Literature Review
2.1 Introduction
2.2 Cell Manipulation Technologies
2.2.1 Optical Tweezers
2.2.2 Magnetic
2.2.3 DEP
2.2.4 Mechanical
2.3 Microfabrication Techniques
2.3.1 2D Planar Electrode Fabrication
2.3.2 3D Electrode Fabrication
2.4 Different Electrodes for DEP Manipulation
2.4.1 2D Planar Microelectrodes
2.4.2 3D Microelectrodes
2.5 Conclusion
Chapter 3 Development of Multilayer Scaffold for 3D Cell Manipulation andPatterning
3.1 Introduction
3.2 Materials and Methods
3.2.1 Multilayer Scaffold Structure Design
3.2.2 Scaffold Fabrication and Assembly
3.2.3 Hardware Setup
3.3 Results and Discussions
3.3.1 Effect of Supply Voltage Input on Multilayer Structure
3.3.2 Characterization of 3D Cellular Pattern
3.3.3 Biocompatibility of Scaffold for Cell Culture
3.3.4 Assessment of Cell Viability after Cell Patterning by DEP
3.3.5 Assessment of Cellular Pattern after Culture
3.4 Conclusions
Chapter 4 Characterization of Honeycomb-patterned Scaffold with DEP-basedPatterning for Bone TE
4.1 Introduction
4.2 Materials and Methods
4.2.1 Scaffold Design
4.2.2 Fabrication
4.2.3 Cell Culture
4.2.4 Cell Preparation for DEP Manipulation
4.2.5 Experimental Setup for DEP-based Cell Patterning
4.3 Results and Discussion
4.3.1 Effects of Input Voltage Frequency
4.3.2 Effects of Material on Cell Culture
4.3.3 Cell Viability during DEP Patterning
4.3.4 Patterning Efficiency
4.4 Conclusions
Chapter 5 Development of Engineered Bone Scaffolds with DEP-basedPatterning utilizing 3D Printing
5.1 Introduction
5.2 Materials and Methods
5.2.1 Scaffold Design
5.2.2 Scaffold Fabrication
5.2.3 Cell Preparation
5.2.4 DEP Cell Patterning
5.2.5 Bone Nodule Formation
5.3 Results and Discussion
5.3.1 Scaffold Characterization
5.3.2 DEP Patterning
5.3.3 Bone Nodule formation
5.4 Conclusions
Chapter 6 Conclusion and Future Work
6.1 Conclusion
6.2 Future Work
Chapter 7 結(jié)束語
7.1 主要工作與貢獻(xiàn)
7.2 研究前景和展望
Reference
致謝
在讀期間發(fā)表的學(xué)術(shù)論文與取得的其他研究成果
中文簡介
本文編號：3979862
【文章頁數(shù)】：112 頁
【學(xué)位級別】：博士
【文章目錄】：
摘要
ABSTRACT
Chapter 1 Introduction
1.1 Background
1.2 Statement of Problems
1.3 Research Objectives
1.4 Methodology and Significance
1.5 Conclusion
Chapter 2 Literature Review
2.1 Introduction
2.2 Cell Manipulation Technologies
2.2.1 Optical Tweezers
2.2.2 Magnetic
2.2.3 DEP
2.2.4 Mechanical
2.3 Microfabrication Techniques
2.3.1 2D Planar Electrode Fabrication
2.3.2 3D Electrode Fabrication
2.4 Different Electrodes for DEP Manipulation
2.4.1 2D Planar Microelectrodes
2.4.2 3D Microelectrodes
2.5 Conclusion
Chapter 3 Development of Multilayer Scaffold for 3D Cell Manipulation andPatterning
3.1 Introduction
3.2 Materials and Methods
3.2.1 Multilayer Scaffold Structure Design
3.2.2 Scaffold Fabrication and Assembly
3.2.3 Hardware Setup
3.3 Results and Discussions
3.3.1 Effect of Supply Voltage Input on Multilayer Structure
3.3.2 Characterization of 3D Cellular Pattern
3.3.3 Biocompatibility of Scaffold for Cell Culture
3.3.4 Assessment of Cell Viability after Cell Patterning by DEP
3.3.5 Assessment of Cellular Pattern after Culture
3.4 Conclusions
Chapter 4 Characterization of Honeycomb-patterned Scaffold with DEP-basedPatterning for Bone TE
4.1 Introduction
4.2 Materials and Methods
4.2.1 Scaffold Design
4.2.2 Fabrication
4.2.3 Cell Culture
4.2.4 Cell Preparation for DEP Manipulation
4.2.5 Experimental Setup for DEP-based Cell Patterning
4.3 Results and Discussion
4.3.1 Effects of Input Voltage Frequency
4.3.2 Effects of Material on Cell Culture
4.3.3 Cell Viability during DEP Patterning
4.3.4 Patterning Efficiency
4.4 Conclusions
Chapter 5 Development of Engineered Bone Scaffolds with DEP-basedPatterning utilizing 3D Printing
5.1 Introduction
5.2 Materials and Methods
5.2.1 Scaffold Design
5.2.2 Scaffold Fabrication
5.2.3 Cell Preparation
5.2.4 DEP Cell Patterning
5.2.5 Bone Nodule Formation
5.3 Results and Discussion
5.3.1 Scaffold Characterization
5.3.2 DEP Patterning
5.3.3 Bone Nodule formation
5.4 Conclusions
Chapter 6 Conclusion and Future Work
6.1 Conclusion
6.2 Future Work
Chapter 7 結(jié)束語
7.1 主要工作與貢獻(xiàn)
7.2 研究前景和展望
Reference
致謝
在讀期間發(fā)表的學(xué)術(shù)論文與取得的其他研究成果
中文簡介
本文編號：3979862
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