發(fā)布時間：2018-04-26 07:18

  本文選題：LED + 黃瓜　； 參考：《甘肅農(nóng)業(yè)大學》2016年博士論文

【摘要】：為了研究補光時間與光質(zhì)對黃瓜(Cucumis sativus L.)幼苗生長和光合特性的影響的影響,以不補光為對照,分析了補光時間(2h,4h和8h)及光質(zhì)[紅藍復(fù)合光(R:B=7:3,R:B=8:2)、藍光(B)、紅光(R)和白光(W)]對黃瓜幼苗形態(tài)學指標、根系活力、抗氧化酶系統(tǒng)、葉綠素熒光參數(shù)、光合速率等參數(shù)的影響,主要研究結(jié)果如下:(1)補照4h的紅藍復(fù)合光(7R/3B)有利于黃瓜幼苗地上部分的生長及干物質(zhì)的積累。補照4h的紅藍復(fù)合光(7R/3B)能有效促進了黃瓜幼苗植株地上部分縱向生長和莖粗的增加。隨著補光處理時間的延長,補光時間及光質(zhì)對黃瓜幼苗葉面積生長的促進作用逐漸顯現(xiàn),補光4h處理及補照紅藍復(fù)合光對黃瓜幼苗葉面積及地上部干物重的促進效果最為明顯。(2)補光時間及光質(zhì)對黃瓜幼苗根長、根表面積、根分枝數(shù)、根系活力及壯苗指數(shù)影響顯著,且互作效應(yīng)顯著。補充4h紅藍復(fù)合光(7:3)可顯著提高根系活力,進而有效促進了黃瓜幼苗干物質(zhì)的積累,為培育壯苗奠定了生理基礎(chǔ)。補光4h處理黃瓜各項生長指標分別優(yōu)于補光2h、8h和不補光處理;補照紅藍復(fù)合光處理黃瓜各項生長指標分別優(yōu)于藍光、紅光、白光和不補光處理。其中,4h補照7R/3B處理下黃瓜幼苗根長、根表面積、根分枝數(shù)和根系活力最高,分別達到1028.0 cm/株、124.1 cm-2/株、5165.3個/株和481.9 mg g-1 h-1;黃瓜幼苗根干重達到0.072g株-1,顯著高于補光2h藍、紅、白光和補光8h藍、紅、白光處理及對照。黃瓜幼苗地上部干重、根干重均與根系活力呈顯著的正相關(guān)關(guān)系(P0.01)。(3)補照4h藍光可以明顯提高黃瓜幼苗葉片可溶性蛋白的含量及保護酶(POD、SOD、APX和CAT)活性,并且使MDA的含量保持相對較低的水平,從而可以有效延緩葉片衰老。與對照相比,補光可顯著提高黃瓜幼苗葉片葉綠素a、葉綠素b、總?cè)~綠素和類胡蘿卜素含量,其中以補光4h紅藍復(fù)合光處理(7R/3B和8R/2B)效果最為顯著;補照不同光質(zhì)可提高黃瓜幼苗可溶性蛋白的含量,其中,以藍光效果最好。(4)補光時間與光質(zhì)對黃瓜幼苗葉片葉綠熒光參數(shù)的影響均明顯表現(xiàn)出處理時間的累加效應(yīng)。紅藍復(fù)合光處理條件下,黃瓜幼苗葉片葉綠素Fv/Fm、ΦPSII和qP值均高于藍光、紅光處理和對照,而NPQ最低。補光時間與光質(zhì)對黃瓜幼苗葉片凈光合速率影響主要體現(xiàn)在一日內(nèi)的10:00至16:00,峰值均出現(xiàn)在12:00。適宜的補光時間及光質(zhì)組合通過提高黃瓜幼苗葉片葉綠素含量和根系活力,增加葉面積,從而提高黃瓜幼苗葉片凈光合速率。(5)不同補光時間及光質(zhì)對黃瓜幼苗葉片SPAD存在顯著影響,且互作效應(yīng)顯著。補光時間與光質(zhì)對黃瓜幼苗葉片SPAD的影響存在時間上的疊加效應(yīng);不同補光時間與光質(zhì)處理條件下,黃瓜幼苗葉色的變化可以表征葉綠素含量的變化。延長光照時間和補照紅藍復(fù)合光、藍光、紅光和白光在一定程度上提高了黃瓜葉片SPAD值。補光時間和光質(zhì)對黃瓜幼苗葉綠體超微結(jié)構(gòu)產(chǎn)生了明顯影響;補照紅光和藍光條件下,淀粉顆粒數(shù)量增多,補照紅藍復(fù)合光葉綠體結(jié)構(gòu)良好,線粒體豐富,基粒、基質(zhì)片層清晰。
[Abstract]:In order to study the effect of light time and light quality on the growth and photosynthetic characteristics of Cucumber (Cucumis sativus L.) seedlings, the morphological index, root activity and antioxidant enzyme system of cucumber seedlings were analyzed by the light supplement (2h, 4H and 8h) and light quality [red blue composite light (R:B=7:3, R:B=8:2), blue light (B), red light (R) and white light (W)]. The main results were as follows: (1) the red and blue composite light (7R/3B) of 4H was beneficial to the growth of the aboveground part of the cucumber seedlings and the accumulation of dry matter. The supplementation of the red and blue light (7R/3B) of 4H could effectively promote the growth of the upper part of the cucumber seedlings and the increase of the stem diameter. The effect of light supplementation time and light quality on leaf area growth of cucumber seedlings gradually appeared with the prolongation of light treatment time. The effect of supplementing light 4H treatment and supplementing red and blue light on leaf area and dry matter weight of cucumber seedlings was the most obvious. (2) the time and light quality of the cucumber seedling root length, root surface area, root branch number and root system The vitality and seedling index were significantly affected, and the interaction effect was significant. Supplementing 4H red and blue light (7:3) could significantly increase the root activity, and thus effectively promote the accumulation of dry matter in cucumber seedlings, and lay a physiological basis for the cultivation of the strong seedlings. The growth index of the supplemental 4h treatment of cucumber is superior to the supplement 2h, 8h and non light treatment, and the combination of red and blue and blue. The growth indexes of light treated cucumber were superior to blue light, red light, white light and non light treatment. Among them, the root length, root surface area, root branch number and root vitality of Cucumber Seedlings under 4H supplementation 7R/3B were the highest, which reached 1028 cm/, 124.1 cm-2/, 5165.3 / 481.9 mg g-1 H-1, and the root dry weight of cucumber seedlings reached -1 of 0.072g strain. Higher than 2H blue, red, white and light 8h blue, red and white light treatment and control. The dry weight and root dry weight of cucumber seedlings have significant positive correlation with root activity (P0.01). (3) the content of soluble protein in cucumber leaves and the activity of protective enzymes (POD, SOD, APX and CAT) in cucumber seedlings can be significantly improved, and the content of MDA is guaranteed. Compared with the control, the content of chlorophyll a, chlorophyll b, total chlorophyll and carotenoid content were significantly increased in the leaves of cucumber seedlings compared with the control. The effect of supplementing light 4H red blue light treatment (7R/3B and 8R/2B) was the most significant, and different light quality could improve the soluble egg of Cucumber seedlings. The effect of blue light was the best in the content of white, and (4) the effect of the time and light quality on the chlorophyll fluorescence parameters of the leaves of cucumber seedlings showed a cumulative effect. Under the condition of red and blue light treatment, the chlorophyll Fv/Fm, PSII and qP values of cucumber seedlings were higher than those of blue light, red light treatment and control, while NPQ was the lowest. The effect of light quality on the net photosynthetic rate of cucumber seedlings was mainly reflected from 10:00 to 16:00 in one day. The peak value of the peak was in the appropriate time of 12:00. and the combination of light quality. The leaf area was increased by increasing the chlorophyll content and root activity of cucumber seedlings, and the net photosynthetic rate of the leaves of yellow melon seedlings was increased. (5) different light filling time. The effect of light quality on the leaf SPAD of cucumber seedlings was significant, and the interaction effect was significant. The effect of light time and light quality on the leaf SPAD of cucumber seedlings had a time superposition effect. Under the different light time and light quality, the change of leaf color of cucumber seedlings could represent the change of chlorophyllin content. Blue light, blue light, red light and white light increased the SPAD value of cucumber leaves to a certain extent. The time and light quality of the cucumber seedlings had an obvious influence on the ultrastructure of the chloroplasts of cucumber seedlings; the amount of starch granules increased under the conditions of red and blue light, and the structure of the red and blue compound light leaf green body was good, the mitochondria were rich, the granules and the matrix layer were clear. Clear.

【學位授予單位】：甘肅農(nóng)業(yè)大學
【學位級別】：博士
【學位授予年份】：2016
【分類號】：S626;S642.2
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本文編號：1805032