ȥøSIRT6֬(x)Ǡ(x)еüC(j)о
l(f)r(sh)g2020-12-03 11:01
ڷֺ2ȴxԼ,֬xl(f)Ɂy,ڸK֬ȽMЕ(hu)F(xin)ǽͽ֬|(zh)ϳɮ֬KҪĴx,ǙC(j)wxƽҪMɲxԼνMp,δޏ(f){(dio)(ji)(do)¸wSliver fibrosis(dng)Sirtuinеһ(g)ҪɆT,Sirtuin6SIRT6,c{(dio)˰x,װY˥ϵSҪ֬MxƽKl(f)l(f)չ^(gu)е߀ǺĿıоMʹÏV(yng)ڻóCre-loxpϵy(tng)ɷNSIRT6֬(x)óСģ,оSIRT6֬(x)еĹ;øǠ(x)ļ(x)ϵ-LX2(x)ԼSIRT6^(gu)_(d)ȫԣD(zhun)СTgSIRT6о(du),оSIRT6ڸǠ(x)еĵá1.оSIRT6֬(x)еã1Ϻ60%֬ιB(yng)ҰСwild-type,WT,Сģ,ͨ^(gu)Western blotzy(c)SIRT6֬Mеı_(d)2SIRT6 floxed(Sirt6...
(li)Դl(xing)t(y)W(xu)Ժʡ
(y)(sh)74 (y)
W(xu)λ(j)eTʿ
ֈDġ
SIRT6FKOС͌(du)СBATMУUCP1PGC1PPARßı_(d)n=3ֻ/M
42 LX2 (x) SIRT6 ı_(d)A10ng/mL TGF1 ̎ LX2 mRNA ˮƽB10ng/mL TGF1 ̎ LX2 (x) 24 hWester_(d)Լ H3K9H3K56 ˮƽ(sh)(j)ʾ(sh)±ƽ(bio)(zhn)` Western blotting zy(c)ٲ SIRT6 ٲ shSIRT6 LknockdownSIRT6 Чʺ Western blotting Y(ji)@ʾD 2-1A Bٲ SIRT
SIRT6 õͶ(do)wSP(gun)ı_(d)D2-2BD 2-2LX2 (x) SIRT6 (du)wSP(gun){(dio)ASIRT6 ͌(du) GFP ٲȾ LX2(x) 24 h ʹûʹ 10ng/mL TGF1 T(do) 24 hwSP(gun)ı_(d)BshSIRT6 ͌(du) shGFP ٲȾ LX2 (x) 24 h ʹûʹ 10ng/mL TGF1 T(do) 24 hwSP(gun)ı_(d)(sh)(j)ʾ(sh)±ƽ(bio)(zhn)`S.E.M*P< 0.05AB
īI(xin)
ڿՓ
[1]Cellular and molecular mechanisms in the pathogenesis of liver fibrosis:An update[J]. Glsm ?zlem Elpek. World Journal of Gastroenterology. 2014(23)
ľ̖(ho)2896304
(li)Դl(xing)t(y)W(xu)Ժʡ
(y)(sh)74 (y)
W(xu)λ(j)eTʿ
ֈDġ
SIRT6FKOС͌(du)СBATMУUCP1PGC1PPARßı_(d)n=3ֻ/M
42 LX2 (x) SIRT6 ı_(d)A10ng/mL TGF1 ̎ LX2 mRNA ˮƽB10ng/mL TGF1 ̎ LX2 (x) 24 hWester_(d)Լ H3K9H3K56 ˮƽ(sh)(j)ʾ(sh)±ƽ(bio)(zhn)` Western blotting zy(c)ٲ SIRT6 ٲ shSIRT6 LknockdownSIRT6 Чʺ Western blotting Y(ji)@ʾD 2-1A Bٲ SIRT
SIRT6 õͶ(do)wSP(gun)ı_(d)D2-2BD 2-2LX2 (x) SIRT6 (du)wSP(gun){(dio)ASIRT6 ͌(du) GFP ٲȾ LX2(x) 24 h ʹûʹ 10ng/mL TGF1 T(do) 24 hwSP(gun)ı_(d)BshSIRT6 ͌(du) shGFP ٲȾ LX2 (x) 24 h ʹûʹ 10ng/mL TGF1 T(do) 24 hwSP(gun)ı_(d)(sh)(j)ʾ(sh)±ƽ(bio)(zhn)`S.E.M*P< 0.05AB
īI(xin)
ڿՓ
[1]Cellular and molecular mechanisms in the pathogenesis of liver fibrosis:An update[J]. Glsm ?zlem Elpek. World Journal of Gastroenterology. 2014(23)
ľ̖(ho)2896304
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