發(fā)布時(shí)間：2018-06-16 19:34

  本文選題：滅活疫苗 + 甲肝病毒　； 參考：《吉林大學(xué)》2006年碩士論文

【摘要】：我國(guó)是肝炎大國(guó),人群的甲肝感染率很高,屬甲肝流行的高發(fā)區(qū)。預(yù)防甲肝最安全和有效的措施是接種甲肝疫苗。當(dāng)前使用的疫苗共有2種類型:甲肝減毒疫苗和甲肝滅活疫苗。國(guó)內(nèi)普遍使用甲肝減毒疫苗,而國(guó)外使用的全部為滅活疫苗。本課題是將甲肝患者糞便中分離的甲型肝炎病毒,在Vero細(xì)胞中進(jìn)行適應(yīng)性培養(yǎng),選肓出高滴度的適應(yīng)株,用于甲肝滅活疫苗研究。用選肓出的甲肝病毒毒株在Vero細(xì)胞中連續(xù)傳代,檢測(cè)其抗原滴度和感染性滴度,滿意后可用此做毒種生產(chǎn)甲肝滅活疫苗。本實(shí)驗(yàn)選用的BL-08株,經(jīng)各項(xiàng)理化實(shí)驗(yàn)證實(shí)為甲型肝炎病毒,該病毒經(jīng)Vero細(xì)胞9次適應(yīng)性傳代以后,病毒滴度高達(dá)2048,感染性滴度為7.33 LogCCID50/ml,初步證明可做為甲型肝炎滅活疫苗的候選毒株.
[Abstract]:Our country is a big country of hepatitis, the infection rate of hepatitis A is very high, belong to the high incidence area of hepatitis A. The safest and most effective way to prevent hepatitis A is to vaccinate against it. There are two types of vaccines currently in use: attenuated hepatitis A vaccine and inactivated hepatitis A vaccine. Hepatitis A attenuated vaccine is widely used in China, while inactivated vaccine is used abroad. In this study, hepatitis A virus isolated from feces of patients with hepatitis A was cultured adaptively in Vero cells, and high titer adaptive strains were selected for the study of inactivated hepatitis A vaccine. The antigenic titer and infectious titer of the selected HAV strain were continuously subcultured in Vero cells and the inactivated hepatitis A vaccine could be produced by using this vaccine. The selected BL-08 strain was confirmed to be hepatitis A virus by various physical and chemical experiments. The virus was subcultured by Vero cells for 9 times. The titer of the virus was as high as 2048 and the infectious titer was 7.33 LogCCID 50 / ml, which was proved to be a candidate for inactivated hepatitis A vaccine.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

【共引文獻(xiàn)】

相關(guān)期刊論文 前7條

1 胡孟冬;;甲型肝炎病毒的組織培養(yǎng):特點(diǎn)、方法和應(yīng)用[J];國(guó)外醫(yī)學(xué).生物制品分冊(cè);1983年02期

2 李琦涵;姜莉;董德祥;曾逸云;;甲型肝炎病毒的組織培養(yǎng)特性[J];國(guó)外醫(yī)學(xué).預(yù)防.診斷.治療用生物制品分冊(cè);1989年02期

3 吳慎,鞠連才,邵惠訓(xùn),張習(xí)坦,孟慶海,李學(xué)光;應(yīng)用A_(549)細(xì)胞分離到1株致細(xì)胞病變的甲型肝炎病毒[J];解放軍醫(yī)學(xué)雜志;1985年03期

4 胡孟冬;R.Scheid;F.Deinhardt;;從中國(guó)病人糞便標(biāo)本中分離到一株甲型肝炎病毒[J];上海醫(yī)學(xué);1982年05期

5 毛江森;謝汝英;黃柏章;陳念良;余佩華;丁占初;;甲型肝炎病毒在一株傳代細(xì)胞(MERN株)中的增殖[J];微生物學(xué)報(bào);1984年01期

6 孟繼鴻;張建瓊;林陵;;輪狀病毒新敏感細(xì)胞的篩選[J];中國(guó)病毒學(xué);1993年01期

7 劉光第;秦賢;張知今;胡克非;趙汝欽;呂麗英;崔淑清;;從出現(xiàn)黃疸后五天的甲型肝炎患者糞便中分離到一株甲型肝炎病毒[J];公共衛(wèi)生與疾病控制雜志;1984年01期

相關(guān)博士學(xué)位論文 前1條

1 井申榮;微載體制備甲肝滅活疫苗的研究[D];中國(guó)協(xié)和醫(yī)科大學(xué);2000年

相關(guān)碩士學(xué)位論文 前1條

1 仲宇航;凍干甲型肝炎—腮腺炎聯(lián)合疫苗凍干保護(hù)劑配比的研究[D];吉林大學(xué);2008年

，

本文編號(hào)：2027867