發(fā)布時間：2018-03-10 09:30

  本文選題：金線蓮多糖　切入點：免疫抑制　出處：《中國獸醫(yī)學(xué)報》2017年07期 　論文類型：期刊論文

【摘要】：50只雄性昆明小鼠適應(yīng)性飼養(yǎng)1周后隨機分為5組,分別為空白對照組、模型組及金線蓮多糖(ARP)低、中、高劑量組,每組10只;第1~3天,除空白對照組注射生理鹽水外,其他各組腹腔注射環(huán)磷酰胺(CTX)80mg/kg,連續(xù)3d;第4~10天,空白組和模型組灌服蒸餾水,其他組分別灌服100,200,400mg/kg的ARP。末次給藥24h后,采集脾臟,qRT-PCR檢測試驗小鼠脾臟細胞因子(IL-2、IFN-γ、IL-4、IL-6)及轉(zhuǎn)錄因子(T-bet、GATA-3)mRNA表達。結(jié)果顯示:與空白對照組相比,模型組IL-2、GATA-3 mRNA表達量降低,IL-4、IL-6、IFN-γ、T-bet mRNA表達量升高;與模型組相比,ARP高劑量組小鼠IL-4mRNA相對表達量降低(P0.05),中、高劑量組IL-6、IFN-γmRNA相對表達量降低(P0.05),ARP組T-bet的相對表達量降低(P0.05),高劑量組GATA-3的相對表達量升高(P0.05)。結(jié)果表明:ARP通過上調(diào)或下調(diào)小鼠脾臟細胞因子與轉(zhuǎn)錄因子的表達,調(diào)節(jié)免疫抑制小鼠的免疫功能。
[Abstract]:After one week of adaptive feeding, 50 male Kunming mice were randomly divided into 5 groups: blank control group, model group and AARP group with low, medium and high doses, 10 rats in each group. The other groups were intraperitoneally injected with CTX 80 mg / kg of cyclophosphamide for 3 days. On the 4th day, the blank group and the model group were given distilled water, and the other groups were given 100 ~ 200g / kg ARP of 400 mg / kg respectively. 24 hours after the last administration, The expression of IL-2mil and T-beta-GATA-3T mRNA were detected by qRT-PCR. The results showed that compared with the control group, the expression of IL-2tGATA-3 mRNA in the model group was significantly lower than that in the control group, and the expression of IL-4 IL-6IFN- 緯 T-bet mRNA in the model group was higher than that in the control group. Compared with the model group, the relative expression of IL-4mRNA in the high dose group was lower than that in the model group. The relative expression of T-bet was decreased in high dose group (P 0.05), and increased in high dose group (P 0.05). The results showed that the expression of spleen cytokines and transcription factors were up-regulated or down-regulated. To regulate the immune function of immunosuppressive mice.
【作者單位】： 福建農(nóng)林大學(xué)中西獸醫(yī)結(jié)合與動物保健福建省高校重點實驗室;
【基金】：國家自然科學(xué)基金資助項目(31372474)
【分類號】：S853.74

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本文編號：1592744