發(fā)布時(shí)間：2018-03-10 22:27

  本文選題：綿羊　切入點(diǎn)：抗菌肽　出處：《中國(guó)畜牧獸醫(yī)》2017年09期 　論文類(lèi)型：期刊論文

【摘要】：為了研究綿羊抗菌肽NK-Lysin主要活性區(qū)的生物學(xué)功能,試驗(yàn)設(shè)計(jì)并合成了3對(duì)功能區(qū)多肽片段,并利用徑向擴(kuò)散試驗(yàn)和最小抑菌濃度對(duì)其抗菌活性進(jìn)行檢測(cè),分析了合成多肽對(duì)雞血紅細(xì)胞的毒性作用,篩選出抗菌效果最好的多肽對(duì)沙門(mén)氏菌攻毒的雛雞進(jìn)行治療,檢測(cè)其治療效果。結(jié)果表明,合成多肽對(duì)大腸桿菌、沙門(mén)氏菌具有抑制活性,多肽片段長(zhǎng)度、多肽C-端有無(wú)酰胺化及多肽內(nèi)的二硫鍵是否成環(huán)對(duì)多肽的抗菌活性均有影響;篩選出的2個(gè)多肽在治療雛雞沙門(mén)氏菌攻毒的過(guò)程中能夠明顯降低死亡率,對(duì)雛雞心臟、肝臟、腎臟的病理?yè)p傷也明顯小于對(duì)照組。本研究結(jié)果為綿羊抗菌肽NK-Lysin作為候選抗菌藥物的開(kāi)發(fā)奠定了基礎(chǔ)。
[Abstract]:In order to study the biological function of the main active regions of sheep antimicrobial peptide NK-Lysin, three pairs of functional peptide fragments were designed and synthesized, and their antibacterial activities were determined by radial diffusion test and minimal inhibitory concentration. The toxic effect of synthetic polypeptides on chicken blood red blood cells was analyzed. The best antibacterial peptides were selected to treat the chickens infected with Salmonella. The results showed that the synthetic polypeptides were effective on Escherichia coli. Salmonella had inhibitory activity, length of peptide fragment, amidation of C-terminal and cyclization of disulfide bond. The two peptides could significantly reduce the mortality during the treatment of salmonella in chicks, and could significantly reduce the mortality of the heart and liver of chicks. The results of this study laid a foundation for the development of sheep antimicrobial peptide NK-Lysin as a candidate antimicrobial agent.
【作者單位】： 石河子大學(xué)動(dòng)物科技學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31360589) 兵團(tuán)博士資金專(zhuān)項(xiàng)(2014BB016) 人社部留學(xué)回國(guó)人員啟動(dòng)(RSLX201304)
【分類(lèi)號(hào)】：S859.79

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