外源褪黑素通過改變抗氧化酶活性和光合作用相關(guān)基因的表達(dá)改善咖啡干旱脅迫耐受性
發(fā)布時(shí)間：2020-12-22 13:56
咖啡是僅次于石油的世界第二大貿(mào)易商品,深受世界各國消費(fèi)者的喜愛�？Х葘儆谲绮菘浦参�,全球約有124種,其中栽培咖啡主要包含小�？Х龋–offea arabica）、大�？Х龋–.liberica）和中粒咖啡(C.canephora三種類型。為了緩解世界人口日益增長的壓力,穩(wěn)定糧食生產(chǎn)可持續(xù)發(fā)展尤為重要。然而,耕地面積的限制以及干旱、鹽堿、極端溫度和生物脅迫等眾多因素對(duì)糧食生產(chǎn)構(gòu)成了嚴(yán)重威脅。因此,提高作物對(duì)不利生長環(huán)境的耐受性對(duì)于確保作物高產(chǎn)穩(wěn)產(chǎn)非常重要。干旱脅迫是影響農(nóng)業(yè)生產(chǎn)的主要非生物脅迫因子之一。對(duì)于咖啡而言干旱脅迫已構(gòu)成影響產(chǎn)量的主要環(huán)境因子,嚴(yán)重時(shí)能導(dǎo)致咖啡產(chǎn)量下降約80%。因此,提高咖啡樹體的抗旱能力是保持咖啡穩(wěn)產(chǎn)的重要條件。褪黑素對(duì)植物在非生物脅迫和生物脅迫下所表現(xiàn)出的生理生化變化的調(diào)節(jié)作用已被廣泛研究,但其對(duì)咖啡植物的作用及機(jī)制卻鮮有研究。為此,本研究探索了外源施加褪黑素對(duì)小�？Х饶秃的芰Φ挠绊�,以期為改善咖啡抗旱性提供理論基礎(chǔ)。主要研究結(jié)果如下:1.葉面噴施或土壤噴施100μM的褪黑素能明顯提高咖啡樹體的抗旱能力;2.干旱脅迫能加速咖啡葉片中葉綠素降解、電解質(zhì)滲漏、脂...
【文章來源】：中國科學(xué)院大學(xué)(中國科學(xué)院武漢植物園)湖北省
【文章頁數(shù)】：72 頁
【學(xué)位級(jí)別】：碩士
【文章目錄】：
摘要
Abstract
List of abbreviations
Chapter one Introduction
1.1 Background
1.2 Coffee genetic diversity
1.3 History of coffee consumption
1.4 Stress
1.5 Effects of drought stress in plants
1.5.1 ROS Scavenging
1.5.2 Enzymatic Antioxidant enzymes
1.6 Melatonin
1.7 Chlorophyll a fluorescence
1.8 Problem statement
1.9 Justification of the study
1.10 General objectives
Chapter two Materials and Methods
2.1 Plant materials
2.2 Methodology
2.2.1 Estimation of total chlorophyll content
2.2.2 Chlorophyll fluorescence
2.2.3 Electrolyte Leakage
2.2.4 Measurement of MDA content
2.2.5 Antioxidant Enzymes Assay
2.2.6 Soluble Protein Content
2.2.7 RNA Extraction
2.2.8 Real-time quantitative PCR （qRT-PCR） analysis
2.3 Statistical analysis
Chapter three Results
3.1 Effects of exogenous melatonin treatment on chlorophyll content
3.2 Effect of melatonin treatment on photosynthetic efficiency under drought stress
3.3 Effect of melatonin treatment on MDA content
3.4 Effects of melatonin treatment on Electrolyte leakage （EL）
3.5 Effect of Melatonin on enzymatic antioxidant enzymes
3.6 Effect of Melatonin on soluble protein content
3.7 Effect of melatonin on relative gene expression of PAO, RBSC2, AREB
Chapter four Discussion
Chapter five Conclusion and Prospects
References
Appendix
Acknowledgement
Author profile and publications
本文編號(hào)：2931879
【文章來源】：中國科學(xué)院大學(xué)(中國科學(xué)院武漢植物園)湖北省
【文章頁數(shù)】：72 頁
【學(xué)位級(jí)別】：碩士
【文章目錄】：
摘要
Abstract
List of abbreviations
Chapter one Introduction
1.1 Background
1.2 Coffee genetic diversity
1.3 History of coffee consumption
1.4 Stress
1.5 Effects of drought stress in plants
1.5.1 ROS Scavenging
1.5.2 Enzymatic Antioxidant enzymes
1.6 Melatonin
1.7 Chlorophyll a fluorescence
1.8 Problem statement
1.9 Justification of the study
1.10 General objectives
Chapter two Materials and Methods
2.1 Plant materials
2.2 Methodology
2.2.1 Estimation of total chlorophyll content
2.2.2 Chlorophyll fluorescence
2.2.3 Electrolyte Leakage
2.2.4 Measurement of MDA content
2.2.5 Antioxidant Enzymes Assay
2.2.6 Soluble Protein Content
2.2.7 RNA Extraction
2.2.8 Real-time quantitative PCR （qRT-PCR） analysis
2.3 Statistical analysis
Chapter three Results
3.1 Effects of exogenous melatonin treatment on chlorophyll content
3.2 Effect of melatonin treatment on photosynthetic efficiency under drought stress
3.3 Effect of melatonin treatment on MDA content
3.4 Effects of melatonin treatment on Electrolyte leakage （EL）
3.5 Effect of Melatonin on enzymatic antioxidant enzymes
3.6 Effect of Melatonin on soluble protein content
3.7 Effect of melatonin on relative gene expression of PAO, RBSC2, AREB
Chapter four Discussion
Chapter five Conclusion and Prospects
References
Appendix
Acknowledgement
Author profile and publications
本文編號(hào)：2931879
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