發(fā)布時(shí)間：2018-03-08 16:28

  本文選題：家禽　切入點(diǎn)：空腸彎曲菌　出處：《浙江農(nóng)業(yè)學(xué)報(bào)》2017年09期 　論文類型：期刊論文

【摘要】：為了解四川地區(qū)禽源空腸彎曲菌(Campylobacter jejuni)的遺傳多樣性和毒力相關(guān)基因的攜帶情況,對(duì)從四川成都、雅安及眉山市采集的雞源、鴨源和鵪鶉源的盲腸及肛拭子樣品進(jìn)行C.jejuni的分離鑒定、ERICPCR分型和17種毒力相關(guān)基因的檢測(cè)分析。結(jié)果顯示,總體分離率為10.23%(48/469),其中雞源分離率顯著高于鵪鶉源(P0.05)。ERIC-PCR聚類分析顯示48株C.jejuni可分為6簇、16類共31種基因型,不同場(chǎng)分離株多在某一類或某一簇內(nèi)聚集分布,而部分菌株在不同地區(qū)、不同場(chǎng)之間呈交叉分布。毒力基因檢測(cè)結(jié)果顯示,15種毒力相關(guān)基因的攜帶率在50%以上,其中鵪鶉源分離株的毒力基因攜帶率高于其他動(dòng)物的攜帶率。由此表明四川地區(qū)禽源C.jejuni毒力基因的攜帶率高并且具有較高的遺傳多樣性。
[Abstract]:In order to understand the genetic diversity and virulence related genes of Campylobacter jejunii (Campylobacter jejunius) collected from Chengdu, Ya'an and Meishan City, Sichuan Province, the genetic diversity and virulence of Campylobacter jejunii were studied. Isolation and identification of C. jejuni from cecum and anal swabs from duck and quail samples by PCR typing and detection of 17 virulence related genes. The total isolation rate was 10.23 / 469%. Among them, the isolation rate of chicken was significantly higher than that of quail P0.05N. ERIC-PCR cluster analysis showed that 48 C. jejuni strains could be divided into 6 clusters, 16 classes and 31 genotypes. Most of the isolates in different fields were clustered in a certain type or cluster, while some strains were clustered in different regions. The results of virulence gene test showed that the carrying rate of 15 virulence related genes was more than 50%. The virulence gene carrying rate of quail isolates was higher than that of other animals, which indicated that C. jejuni virulence gene was higher in Sichuan and had higher genetic diversity.
【作者單位】： 四川農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)院動(dòng)物疫病與人類健康四川省重點(diǎn)實(shí)驗(yàn)室;
【基金】：四川農(nóng)業(yè)大學(xué)學(xué)科建設(shè)雙支計(jì)劃(3572070) 四川省教育廳重點(diǎn)項(xiàng)目(15ZA0024)
【分類號(hào)】：S852.61

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